Recommended Reaction Conditions:

Initial Conditions:

Initial denaturation at start: 92 - 97oC for 3 - 5 min. If you denature at 97oC, denature sample only; add rest of mix after reaction colls to annealing temperature (prevents premature denaturation of enzyme).

Initial annealing temperature: as high as feasible for 3 min (eg: 50 - 75oC). Stringent initial conditions mean less non-specific product, especially when amplifying from eukaryotic genomic DNA.

Initial elongation temperature: 72oC for 3 - 5 min. This allows complete elongation of product on rare templates.

PCR方法相关产品:

• 电泳设备
• 紫外设备
• 普通PCR仪
• 定量PCR仪
• PCR/RT-PCR/qPCR试剂
• PCR引物
• PCR试剂
• PCR对照
• 特异性PCR试剂盒
• PCR克隆试剂盒
• RNA
• RNase检测/去除
• RT-PCR试剂
• RT-PCR标准品
• 定量PCR试剂
• 定量PCR标记
• 总RNA分离纯化盒
• PCR产物纯化
• 核酸酶
• 聚合酶
• 反转录酶