预优化siRNA转染试剂MCF7相关交流-蚂蚁淘在线

预优化siRNA转染试剂MCF7

mayitao /发布时间:1541308909 /浏览量:38

Description:GenMute™siRNATransfectionReagentforMCF7Cellispre-optimizedfortransfectingsiRNAtoMCF7cells. MCF-7cellisabreastcancercelllinewasisolatedin1970froma69-year-oldCaucasianwoman.Thiscelllineretainedseveralcharacteristicsofdifferentiatedmammaryepitheliumincludingtheabilitytoprocessestradiolviacytoplasmicestrogenreceptorsandthecapabilityofformingdomes.ThiscelllineexpressestheoncogeneWnt7B.Tumornecrosisfactoralpha(TNFalpha)inhibitsthegrowthofMCF-7breastcancercells.Treatmentwithanti-estrogenscanmodulatethesecretionofinsulin-likegrowthfactorbindingproteins.Size&content:-GenMute™Reagent,1.0ml,sufficientfor~833reactionsbasedontransfecting20pmolessiRNAormiRNAmimicsin24-wellplate-GenMute™TransfectionBuffer(5x),formulatedformaximaltransfectionefficiency,8.0mltomake40mlworkingsolutionRefertothefollowingoptimaltransfectionconditionsformaximalsilencingonMCF7cells.GenMute™reagent,1.0ml,issufficientfor~833transfectionsin24wellplatesor~416transfectionsin6wellplates.

SummaryofOptimalTransfectionConditions:CellculturemediumConfluencyofcellonthedayoftransfectionOptimalsiRNAconcentrationDiluentforsiRNAandGenMute™ ReagentIncubationTimetoFormGenMute™/siRNAComplexMaximalSilencingEfficiency TransfectionResults:ReporterGeneSilencingEfficiency(%)

DMEMwith4.5g/Lglucose,10%FBS~50%40nM1xGenMute™TransfectionBuffer~15minutesatRT48hoursRenillaLuciferase90%

Storage:Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.

DataSheet&Protocol.pdf

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