Description
GenJet™DNA InVitro TranfectionReagentforM-PACispre-optimizedfortransfectingM-PAC(alsocelledmPAC)cells. M-PACismoderatelyimpregnatedpancreasacinarunitcells,also oneofhard-to-transfectcellline.

RefertothefollowingoptimaltransfectionconditionsformaximaltransfectionefficiencyonM-PACcells.GenJet™reagent,1.0ml,issufficientfor300to600transfectionsin24wellplatesor50to100transfectionsin6wellplates.

SummaryofOptimalTransfectionConditions: Confluenceonthedayoftransfection Cellcultureconditions GenJet™(µl):DNA(µg)Ratio DiluentforDNAandTransfectionReagent IncubationTimetoFormGenJet™/DNAComplex PresenceofSerum/AntibioticsduringTransfection ChangeMediumAfterTransfection MaximalEfficiency  TransfectionResults: ReporterGene Plasmid Efficiency(GFP%)

90% DMEMwith4.5g/Lglucose,10%FBS 3:1 Serum-freeDMEMwith4.5g/Lglucose 15minutesatRT Yes Yes,~5HoursAfterTransfection 48hours EGFP pEGFP-N3(CMVpromoter)  60%

StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger

DataSheet 
 

温馨提示：不可用于临床治疗。