AimToensureallcellcultureproceduresareperformedtoastandardthatwillpreventcontaminationfrombacteria,fungiandmycoplasmaandcrosscontaminationwithothercelllines.

Materials

• Chloros/Preseptsolution(2.5g/l)
• 1%formaldehydebaseddisinfectante.g.Virkon,Tegador
• 70%ethanolinwater(Prod.No.R8382)

Equipment

• Personalprotectiveequipment(sterilegloves,laboratorycoat,safetyvisor)
• MicroBIOLOGicalsafetycABInetatappropriatecontainmentlevel

Procedure

1. Sanitizethecabinetusing70%ethanolbeforecommencingwork.
2. Sanitizeglovesbywashingthemin70%ethanolandallowingtoairdryfor30secondsbeforecommencingwork.
3. Putallmaterialsandequipmentintothecabinetpriortostartingworkaftersanitizingtheexteriorsurfaceswith70%ethanol.
4. Whilstworkingdonotcontaminateglovesbytouchinganythingoutsidethecabinet(especiallyfaceandhair).Ifglovesbecomecontaminatedre-sanitizewith70%ethanolasabovebeforeproceeding.
5. Discardglovesafterhandlingcontaminatedculturesandattheendofallcellcultureprocedures.
6. Equipmentinthecabinetorthatwhichwillbetakenintothecabinetduringcellcultureprocedures(mediabottles,Pipettetipboxes,pipetteaids)shouldbewipedwithtissuesoakedwith70%ethanolpriortouse.
7. Movementwithinandimmediatelyoutsidethecabinetmustnotberapid.Slowmovementwillallowtheairwithinthecabinettocirculateproperly.
8. Speech,sneezingandcoughingmustbedirectedawayfromthecabinetsoasnottodisrupttheairflow.
9. Aftercompletingworkdisinfectallequipmentandmaterialbeforeremovingfromthecabinet.Spraytheworksurfacesinsidethecabinetwith70%ethanolandwipedrywithtissue.Disposeoftissuebyautoclaving.
10. Cellculturediscardinchloros(10,000)ppmmustbekeptinthecabinetforaminimumoftwohours(preferablyovernight)priortodiscardingdownthesinkwithcopiousamountsofwater.
11. PeriodicallycleanthecabinetsurfaceswithadisinfectantsuchasPresept,TegadororVirkonorfumigatethecabinetaccordingtothemanufacturersinstructions.Howeveryoumustensurethatitissafetofumigateyourownlaboratoryenvironmentduetothegenerationofgaseousformaldehyde,consultyouron-siteHealthandSafetyAdvisor.

================  蚂蚁淘在线  ================

免责声明：本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容

版权声明：未经蚂蚁淘在线授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的，应该授权范围内使用，并注明“来源：蚂蚁淘在线”。违反上述声明者，本网将追究其相关法律责任。