Formulation	H2O
MolecularWeight
Storage	-80°C
Purity	HPLCandTLCanalysis
Compound
Assay	N/A
ShelfLife(properlystored)	12months
ChemicalFormula

StructureofDAPA
ThestructureofdansylarginineN-(3-ethyl-1,5-pentanediyl)amide(DAPA)isillustrated.DAPAisapotentinhibitorofthrombinwhichexhibitsuniquechangesinitsfluorescentpropertieswhenboundtothrombin.

Overview:

DansylarginineN-(3-ethyl-1,5-pentanediyl)amide,morecommonlyreferredtoasDAPA,isapotent(Ki=10-7M)andspecificsyntheticthrombininhibitor(1).OfspecialinterestaretheuniquefluorescentpropertiescontributedbythedansylmoietyofDAPA.Whenboundtothrombin,thefluorescenceintensityandlifetimeofthedansylmoietyareincreasedthreefold.Theenhancementoffluorescenceintensity,coupledwithitsinhibitoryproperties,havemadethiscompoundextremelyusefulforstudiesinvolvingthrombingeneration(2-7).Inadditiontoanincreaseinfluorescenceintensityandlifetime,thereisadecreaseindepolarizationoftheexcitationsignalwhichalsosupportsfluorescencepolarizationstudies.

TheinhibitorpropertiesofDAPAalonehavebeenusefulinavarietyofapplications.DAPAisroutinelyusedasaproteaseinhibitorduringtheisolationofproteinsthataresusceptIBLetothrombincleavage.FurThermore,byeliminatingthefeedbackofthrombinactivityduringprothrombinactivation,theisolationofreactionintermediatessuchasfragment1.2,andmeizothrombinhavebeenmadepossible(7).

DAPAissynthesizedaccordingtheproceduredescribedbyNesheim,etal.(1).HPLC,TLCandspectralanalysesareusedtodeterminethepurityofthefinalproduct.DAPAissuppliedinwater.Whenproperlystoredat-20°Candprotectedfromlight,thecompoundisstableformanyyears.

Properties:

Formula	C25H39O3N6S1Cl1(reference#1) DansylarginineN-(3-ethyl-1,5-pentanediyl)amide
Molecularweight	539g/mole(1)
Extinctioncoefficient	E 1% 1cm,330nm =4010(1)
Specialproperties	Aspecificandpotentthrombininhibitor(Ki=10-7M).Exhibitsa3-foldincreaseinfluorescenceintensityandlifetimewhenboundtothrombin.

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