Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pLuc-C1
  (Search Vector Database)
• Backbone manufacturer
  Derived by our lab using pExFP-C1 and pGL3
• Backbone sizew/o insert(bp)5673
• Total vector size (bp)6119
• Vector type
  Mammalian Expression, Luciferase
• Selectable markers
  Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s)
  Kanamycin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  OptoJNKi dark-state mutant
• Alt name
  AsLov2Jalpha (404-546)-C450A-JIP11
• Species
  Synthetic; Avena Sativa and
• Insert Size (bp)
  478
• Mutation
  C450A mutation of AsLov2Jalpha
• GenBank ID
  KY595941
• PromoterCMV
• Tag/ Fusion Protein
  • Firefly luciferase (N terminal on backbone)

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteEcoRI(not destroyed)
• 3′ cloning siteBamHI(not destroyed)
• 5′ sequencing primerluc2131+
• 3′ sequencing primerfastbac reverse
(Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by
  We designed and assembled the insert from de novo synthesised sequences
• Terms and Licenses
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms
  • Not Available to Industry

Depositor Comments