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商品描述
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 20424 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepWZL-Neo-Myr-Flag-DEST(Search Vector Database)
- Backbone manufacturerWilliam Hahn Lab (available at Addgene, #15300)
- Vector typeMammalian Expression, Retroviral
- Selectable markersNeomycin (select with G418)
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Stbl3
- Growth instructionsStbl3 cells to prevent recombination.
- Copy numberUnknown
Gene/Insert
- Gene/Insert nameAMHR2
- Alt nameanti-Mullerian hormone receptor, type II isoform 3
- SpeciesH. sapiens (human)
- MutationC473G mutation when compared to Genbank reference sequence NP_001158163.1
- GenBank ID
- Entrez GeneAMHR2(a.k.a. AMHR, MISR2, MISRII, MRII)
- Tags/ Fusion Proteins
- Myr (N terminal on backbone)
- Flag (N terminal on backbone)
Cloning Information
- Cloning methodGateway Cloning
- 5′ sequencing primerpWZL-Fwd (GAA CCT CCT CGT TCG ACC)
- 3′ sequencing primerpWZL-Rev (TTC CGG GCC CTC ACA TTG) (Common Sequencing Primers)
Resource Information
- A portion of this plasmid was derived from a plasmid made by2-B2
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
Depositor Comments
ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations.Please see the following link for plasmids from this article that are not part of the kinase libraryhttp://www.addgene.org/pubmed/17574021
