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Uscnk/ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)/96T*100/KSA047Hu01

价格
¥163800.00
货号:KSA047Hu01
浏览量:127
品牌:Uscnk
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商品描述

ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)

HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen

  • Cytokine
  • Tumor immunity
  • Hepatology
  • Product No.KSA047Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
    • All
    • Human
    • Mouse
    • Rat
    • Cavia
    • Rabbit
    • Simian
    • Caprine
    • Ovine
    • Equine
    • Bovine
    • Porcine
    • Gallus
    • Canine
    • Others
    • Multi-species
    • Pan-species
  • Reagent ContentsCapture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2000pg/mL
  • Applicable Sensitivity12.3pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • DownloadInstruction Manual
  • UOM96T*596T*1096T*2096T*5096T*100
  • FOBUS$ 819 For more details, please contact local distributors!US$ 1365 For more details, please contact local distributors!US$ 2457 For more details, please contact local distributors!US$ 4778 For more details, please contact local distributors!US$ 8190 For more details, please contact local distributors!
  • ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)Packages (Simulation)
  • ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)Packages (Simulation)
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)

The Abs in the kit have high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor (HGF).No significant cross-reactivity or interference between Hepatocyte Growth Factor (HGF) and analogues was observed.

USAGE of the ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.2. Aspirate and wash 1 time.3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE of the ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

SUPPORT PACK of the ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)

GIVEAWAYS

ELISA / CLIA Experiment Service

INCREMENT SERVICES

CLIA Kit Customized ServiceELISA Kit Customized ServiceAssay Kit DIY Support Pack 1Assay Kit DIY Support Pack 2Standard DiluentCoating BufferDiluent Buffer for AntibodyAssay Kit DIY Support Pack 3Assay Kit DIY Support Pack 4Real Time PCR Experimental Service

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GUESTBOOK
Uscnk睫状神经营养因子(CNTF)的CLIA试剂盒碳纳米管细胞因子神经科学产品编号SCA021Ra生物物种褐家鼠(Rattus)(Rat)同名,不同种。测试方法双抗体三明治测定长度2小时40分钟检测范围6.86-5,000pg / mL灵敏度该试剂盒的最小可检测剂量通常小于2.61pg / mL。样品类型血清,血浆,组织匀浆,细胞培养上清液和其他生物液体下载使用说明书单位48T 96T 96T * 5 96T * 10 96T * 100离岸价US $ 912CLIA试剂盒对睫状神经营养因子(CNTF)的特异性该检测方法对睫状神经营养因子(CNTF)的检测具有很高的灵敏度和出色的特异性。睫状神经营养因子(CNTF)与类似物之间未发现明显的交叉反应或干扰。睫状神经营养因子(CNTF)CLIA试剂盒的回收在下面列出的基质中加入一定水平的重组睫状神经营养因子(CNTF),并通过将测量值与样品中的睫状神经营养因子(CNTF)的预期量进行比较来计算回收率。矩阵 回收率(%) 平均(%)血清(n = 5) 96-105 101EDTA血浆(n = 5) 86-94 89肝素血浆(n = 5) 97-105 101睫状神经营养因子(CNTF)CLIA试剂盒的精确度测定内精密度(测定内精密度):在一块平板上分别对3个具有低,中和高水平睫状神经营养因子(CNTF)的样品进行了20次测试。批间精密度(批间精密度):在3个不同的平板上测试3个具有低,中和高水平睫状神经营养因子(CNTF)的样品,每个平板重复8次。CV(%)= SD /平均值X100批内:CV <10%批间:CV <12%睫状神经营养因子(CNTF)CLIA试剂盒的线性通过测试掺有适当浓度的睫状神经营养因子(CNTF)及其系列稀释液的样品,可以测定试剂盒的线性。通过计算浓度相对于预期浓度的百分比来证明结果。样品 1:2 1:4 1:8 1:16血清(n = 5) 78-96% 80-91% 90-99% 93-101%EDTA血浆(n = 5) 90-101% 79-89% 86-98% 87-95%肝素血浆(n = 5) 78-103% 80-99% 96-105% 96-103%睫状神经营养因子(CNTF)CLIA试剂盒的稳定性试剂盒的稳定性取决于活性的丧失率。在适当的存储条件下,该工具包在有效期内的丢失率小于5%。为了最大程度地减少对性能,操作程序和实验室条件的额外影响,尤其是室温,空气湿度,培养箱温度,应严格控制。还强烈建议整个测试过程由同一操作员从头到尾进行。睫状神经营养因子(CNTF)CLIA试剂盒的测定方法摘要1.准备所有试剂,样品和标准品;2.向每个孔中添加100µL标准液或样品。在37°C下孵育1小时;3.吸出并加入100µL准备好的检测试剂A。在37°C孵育1小时;4.吸净并清洗3次;5.加入100µL准备好的检测试剂B。在37°C孵育30分钟;6.吸气并清洗5次;7.添加100µL底物溶液。在37°C下孵育10分钟;8.立即读取RLU值。睫状神经营养因子(CNTF)CLIA试剂盒的测试原理该试剂盒中提供的微孔板已预先涂有针对睫状神经营养因子(CNTF)的抗体。然后将标准品或样品与对睫状神经营养因子(CNTF)有特异性的生物素偶联抗体一起添加到适当的微孔板孔中。接下来,将缀合至辣根过氧化物酶(HRP)的抗生物素蛋白加入每个微孔板中并孵育。然后加入底物A和B的混合物以产生辉光发射动力学。平板显影后,发射光的强度与样品或标准品中的睫状神经营养因子(CNTF)水平成正比。礼物ELISA / CLIA实验服务