Mini Samples ELISA Kit for Interleukin 13 (IL13)
ALRH; BHR1; P600; IL-13; NC30
- Product No.MEA060Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Rangen/a
- Sensitivityn/a
- Sample Typen/a
- Downloadn/a
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 393 For more details, please contact local distributors!US$ 562 For more details, please contact local distributors!US$ 2529 For more details, please contact local distributors!US$ 4777 For more details, please contact local distributors!US$ 39340 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Mini Samples ELISA Kit for Interleukin 13 (IL13)
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 13 (IL13).No significant cross-reactivity or interference between Mini Samples Interleukin 13 (IL13) and analogues was observed.
Recovery of the Mini Samples ELISA Kit for Interleukin 13 (IL13)
Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 13 (IL13) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 13 (IL13) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-95 | 86 |
| EDTA plasma(n=5) | 86-98 | 89 |
| heparin plasma(n=5) | 97-104 | 101 |
Precision of the Mini Samples ELISA Kit for Interleukin 13 (IL13)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 13 (IL13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 13 (IL13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the Mini Samples ELISA Kit for Interleukin 13 (IL13)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 13 (IL13) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-96% | 94-103% | 85-103% | 97-104% |
| EDTA plasma(n=5) | 84-94% | 81-101% | 94-103% | 80-103% |
| heparin plasma(n=5) | 91-99% | 79-105% | 80-94% | 87-101% |
Stability of the Mini Samples ELISA Kit for Interleukin 13 (IL13)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Mini Samples ELISA Kit for Interleukin 13 (IL13)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the Mini Samples ELISA Kit for Interleukin 13 (IL13)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 13 (IL13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 13 (IL13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 13 (IL13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Interleukin 13 (IL13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
GIVEAWAYS
ELISA / CLIA Experiment Service
INCREMENT SERVICES
Single-component Reagents of Assay KitLysis Buffer Specific for ELISA / CLIAQuality Control of ELISA / CLIADisease Model Customized ServiceTGFB1 Activation ReagentReal Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA060Mu02 | Recombinant Interleukin 13 (IL13) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA060Mu01 | Recombinant Interleukin 13 (IL13) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA060Mu01 | Polyclonal Antibody to Interleukin 13 (IL13) | WB; IHC; ICC; IP. |
| PAA060Mu02 | Polyclonal Antibody to Interleukin 13 (IL13) | WB; IHC; ICC; IP. |
| SEA060Mu | ELISA Kit for Interleukin 13 (IL13) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA060Mu | Mini Samples ELISA Kit for Interleukin 13 (IL13) | Enzyme-linked immunosorbent assay for Antigen Detection. |
