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Uscnk/Active Myostatin (MSTN)/5mg/APB653Hu61

价格
¥232200.00
货号:APB653Hu61
浏览量:127
品牌:Uscnk
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商品描述

Active Myostatin (MSTN)

GDF8; Growth Differentiation Factor 8

  • Cytokine
  • Metabolic pathway
  • Bone metabolism
  • Product No.APB653Hu61
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
    • All
    • Human
    • Mouse
    • Rat
    • Cavia
    • Rabbit
    • Simian
    • Caprine
    • Ovine
    • Equine
    • Bovine
    • Porcine
    • Gallus
    • Canine
    • Others
    • Multi-species
    • Pan-species
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • TraitsFreeze-dried powder
  • Purity> 95%
  • Isoelectric Point6.4
  • ApplicationsCell culture; Activity Assays.
  • DownloadInstruction Manual
  • UOM10µg50µg200µg1mg5mg
  • FOBUS$ 310 For more details, please contact local distributors!US$ 774 For more details, please contact local distributors!US$ 1548 For more details, please contact local distributors!US$ 4644 For more details, please contact local distributors!US$ 11610 For more details, please contact local distributors!
  • Active Myostatin (MSTN)Packages (Simulation)
  • Active Myostatin (MSTN)Packages (Simulation)
  • APB653Hu61.pngFigure. SDS-PAGE
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Myostatin (MSTN)

Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) is a member of the TGF beta protein family. Myostatin is a secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and BMP1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of MSTN with BMP1.

USAGE of the Active Myostatin (MSTN)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Myostatin (MSTN)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Myostatin (MSTN)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service

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GUESTBOOK
Uscnk睫状神经营养因子(CNTF)的CLIA试剂盒碳纳米管细胞因子神经科学产品编号SCA021Ra生物物种褐家鼠(Rattus)(Rat)同名,不同种。测试方法双抗体三明治测定长度2小时40分钟检测范围6.86-5,000pg / mL灵敏度该试剂盒的最小可检测剂量通常小于2.61pg / mL。样品类型血清,血浆,组织匀浆,细胞培养上清液和其他生物液体下载使用说明书单位48T 96T 96T * 5 96T * 10 96T * 100离岸价US $ 912CLIA试剂盒对睫状神经营养因子(CNTF)的特异性该检测方法对睫状神经营养因子(CNTF)的检测具有很高的灵敏度和出色的特异性。睫状神经营养因子(CNTF)与类似物之间未发现明显的交叉反应或干扰。睫状神经营养因子(CNTF)CLIA试剂盒的回收在下面列出的基质中加入一定水平的重组睫状神经营养因子(CNTF),并通过将测量值与样品中的睫状神经营养因子(CNTF)的预期量进行比较来计算回收率。矩阵 回收率(%) 平均(%)血清(n = 5) 96-105 101EDTA血浆(n = 5) 86-94 89肝素血浆(n = 5) 97-105 101睫状神经营养因子(CNTF)CLIA试剂盒的精确度测定内精密度(测定内精密度):在一块平板上分别对3个具有低,中和高水平睫状神经营养因子(CNTF)的样品进行了20次测试。批间精密度(批间精密度):在3个不同的平板上测试3个具有低,中和高水平睫状神经营养因子(CNTF)的样品,每个平板重复8次。CV(%)= SD /平均值X100批内:CV <10%批间:CV <12%睫状神经营养因子(CNTF)CLIA试剂盒的线性通过测试掺有适当浓度的睫状神经营养因子(CNTF)及其系列稀释液的样品,可以测定试剂盒的线性。通过计算浓度相对于预期浓度的百分比来证明结果。样品 1:2 1:4 1:8 1:16血清(n = 5) 78-96% 80-91% 90-99% 93-101%EDTA血浆(n = 5) 90-101% 79-89% 86-98% 87-95%肝素血浆(n = 5) 78-103% 80-99% 96-105% 96-103%睫状神经营养因子(CNTF)CLIA试剂盒的稳定性试剂盒的稳定性取决于活性的丧失率。在适当的存储条件下,该工具包在有效期内的丢失率小于5%。为了最大程度地减少对性能,操作程序和实验室条件的额外影响,尤其是室温,空气湿度,培养箱温度,应严格控制。还强烈建议整个测试过程由同一操作员从头到尾进行。睫状神经营养因子(CNTF)CLIA试剂盒的测定方法摘要1.准备所有试剂,样品和标准品;2.向每个孔中添加100µL标准液或样品。在37°C下孵育1小时;3.吸出并加入100µL准备好的检测试剂A。在37°C孵育1小时;4.吸净并清洗3次;5.加入100µL准备好的检测试剂B。在37°C孵育30分钟;6.吸气并清洗5次;7.添加100µL底物溶液。在37°C下孵育10分钟;8.立即读取RLU值。睫状神经营养因子(CNTF)CLIA试剂盒的测试原理该试剂盒中提供的微孔板已预先涂有针对睫状神经营养因子(CNTF)的抗体。然后将标准品或样品与对睫状神经营养因子(CNTF)有特异性的生物素偶联抗体一起添加到适当的微孔板孔中。接下来,将缀合至辣根过氧化物酶(HRP)的抗生物素蛋白加入每个微孔板中并孵育。然后加入底物A和B的混合物以产生辉光发射动力学。平板显影后,发射光的强度与样品或标准品中的睫状神经营养因子(CNTF)水平成正比。礼物ELISA / CLIA实验服务