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ECM BIOSCIENCES/phospho-Arpc1b/p41-Arc (Thr-21) Peptide/50 μg/AX4355

价格
¥1100.00
货号:AX4355
浏览量:127
品牌:ECM BIOSCIENCES
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商品描述

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. p41-Arc (Arpc1b) subunit Arpc1 has two isoforms in humans, Arpc1a and Arpc1b. PAK1 can bind and phosphorylate Thr-21 in Arpc1b leading to growth factor-stimulated cell motility. In addition, Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on Thr-21 and a nonphosphorylatable Arpc1b mutant cannot activate Aurora A kinase and centrosome amplification. Thus, Arpc1b has roles in cytoskeletal dynamics during cell motility and mitosis, and these activities are regulated by phosphorylation at Thr-21.

 

References

Molli, P.R. et al. (2010) J Cell Biol. 190(1):101. Vadlamudi, R.K. et al. (2004) EMBO Rep. 5(2):154.Welch, M.D. et al. (1997) J Cell Biol. 138(2):375.

Phospho-Arpc1b/p41-Arc (Thr-21) synthetic peptide corresponding to amino acid residues surrounding threonine 21 in human Arpc1b. This peptide sequence is highly conserved in rat, mouse, chicken, and fish Arpc1b, and has some homology to the conserved site in isoform Arpc1a.
*For more information, see UniProt Accession O15143
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-Arpc1b/p41-Arc (Thr-21) phospho-specific antibody (AP4351) in ELISA, and has been shown to block the reactivity of AP4351 during Western blot. In addition, the peptide is recommended for use in blocking AP4351 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY

ECM BIOSCIENCES的A431细胞裂解液A431细胞在细胞表面表达约106种表皮生长因子(EGF)受体。在用EGF刺激后,A431细胞的EGF受体磷酸化水平显着增加,随后激活了主要的细胞信号传导途径,例如PKB / Akt和MAP激酶途径。在EGF刺激后的不同时间点,这些细胞信号通路的下游,各种细胞骨架,细胞质和核蛋白被磷酸化。在含有10%胎牛血清的DMEM中生长的A431细胞的融合培养物在1%SDS,1.0 mM原钒酸钠,10 mM Tris(pH 7.4)缓冲液中裂解。在稀释至最终浓度和缓冲液之前,使用BCA方法(Pierce)确定蛋白质浓度。