Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.
References
Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).
Immunocytochemical labeling of VE-Cadherin in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with rabbit polyclonal VE-Cadherin (a.a. 770-781), then the antibody was detected using appropriate secondary antibody conjugated to Cy3. Phase image (left) and fluorescent image (right).
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
Product References:
CP1981 Kishor, S.K. et al.(2015) Cardiovasc Res. 108(1):171-80 WB: HUVECRS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsMS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western BlotRS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western BlotCP1981 Adam, A.P. et al. (2010) J Biol Chem. 285(10):7045. WB: HDMECsCP1981 Bowers, S. et al. (2010) 1188:143. Cell-Cell interactionsCP1981 Cain, R. J. et al. (2010) J Cell Biol. 188(6):863. WB: HUVECsCP1981 Lo, CW et al. (2010) Cancer Res. 71(2):424. WB: HUVECsCP2231 Bowers, S.L. et al. (2010) 1188:143. FB: negative control in Cell-Cell contact assayThis kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
CP2231 | VE-Cadherin (a.a. 770-781) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 140 |
CM0351 | VE-Cadherin (Extracellular region) Mouse mAb | 50 μl | WB, E, ICC, FC | Hu | 140 |
CP1981 | VE-Cadherin (Tyr-685), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 140 |
MS3001 | Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | |
RS3251 | Anti-Rabbit Ig Light Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
KIT SUMMARY
The VE-cadherin phospho-regulation antibody sampler kit can be used to detect the phosphorylation of Tyr-685 relative to total expression of VE-cadherin. The kit includes a phospho-specific antibody to detect VE-cadherin Tyr-685, and mouse monoclonal and rabbit polyclonal antibodies to examine total VE-cadherin expression. The kit also includes secondary reagents for detection of primary antibodies in various applications.
