CD98 (SLC3A2/4F2 heavy chain) is a transmembrane protein that belongs to the solute carrier family. CD98 forms heterodimeric complexes with various amino acid transporters such as LAT1 and LAT2 and regulates uptake of amino acids. CD98 is an early expressed antigen on the surface of activated human lymphocytes, and is also expressed in most cell types with the exception of platelets. CD98 is highly expressed in the tubules of the kidney and the gastrointestinal tract. CD98 also expressed in tumors, including glioma, ovarian cancer, and astrocytomas. Expression of CD98 has been implicated in tumor progression and correlates with poor outcome in patients with pulmonary neuroendocrine tumors. CD98 is also involved in integrin trafficking through association with β1 and β4 integrins, and regulates keratinocyte adhesion and differentiation. Thus, CD98 is a widely expressed transmembrane protein involved in amino acid transport, integrin function, and differentiation.

 

References

ECM BIOSCIENCES的A431细胞裂解液A431细胞在细胞表面表达约106种表皮生长因子（EGF）受体。在用EGF刺激后，A431细胞的EGF受体磷酸化水平显着增加，随后激活了主要的细胞信号传导途径，例如PKB / Akt和MAP激酶途径。在EGF刺激后的不同时间点，这些细胞信号通路的下游，各种细胞骨架，细胞质和核蛋白被磷酸化。在含有10％胎牛血清的DMEM中生长的A431细胞的融合培养物在1％SDS，1.0 mM原钒酸钠，10 mM Tris（pH 7.4）缓冲液中裂解。在稀释至最终浓度和缓冲液之前，使用BCA方法（Pierce）确定蛋白质浓度。