CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5"-nucleotidase family. CD73 is an ecto 5"Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5"Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer. CD73 has been implicated as a prognostic indicator and therapeutic target in cancers. CD73 may act as an immune checkpoint inhibitor to natural killer cells in tumors, and this activity correlates with tumor invasiveness, metastasis, and poor outcome in cancer patients.

References

ECM BIOSCIENCES的A431细胞裂解液A431细胞在细胞表面表达约106种表皮生长因子（EGF）受体。在用EGF刺激后，A431细胞的EGF受体磷酸化水平显着增加，随后激活了主要的细胞信号传导途径，例如PKB / Akt和MAP激酶途径。在EGF刺激后的不同时间点，这些细胞信号通路的下游，各种细胞骨架，细胞质和核蛋白被磷酸化。在含有10％胎牛血清的DMEM中生长的A431细胞的融合培养物在1％SDS，1.0 mM原钒酸钠，10 mM Tris（pH 7.4）缓冲液中裂解。在稀释至最终浓度和缓冲液之前，使用BCA方法（Pierce）确定蛋白质浓度。