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商品描述
Ubiquitin Linkage Screening Kit II is designed for two purposes: 1) to identify the ubiquitin lysine residue that is used to link the polyubiquitin chain conjugated on a protein substrate; 2) to determine whether a protein substrate is polyubiquitinated or conjugated with multiple monoubiquitin.
Additional Information
| Product Name: | Ubiquitin Linkage Screening Kit II |
|---|---|
| Also Known As: | N/A |
| Catalog No.: | J3210 |
| Size: | 1 Kit |
| Molecular Weight: | N/A |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | 95% based on SDS-PAGE |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF datasheet, MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | N/A |
| Shipping Method: | Dry ice shipping |
| References: | N/A |
Details
* The supplied UBE1 is sufficient for setting up 50 x 20 µl reactions. Each Ub mutant is sufficient for setting up 10 X 20 µl reactions.
Description
Ubiquitin Linkage Screening Kit II is designed for two purposes: 1) to identify the ubiquitin lysine residue that is used to link the polyubiquitin chain conjugated on a protein substrate; 2) to determine whether a protein substrate is polyubiquitinated or conjugated with multiple monoubiquitin.
A typical 20 µl reaction contains 100 nM UBE1, 2 µM E2 (not supplied), 50 µM Ub or Ub mutants, 4 mM ATP, 2 µM E3 (not supplied) and 2 µM substrate (no supplied) in the ubiquitination buffer.
Components
• 20X UBE1 (2 µM)..................................50 µl • 10X 6xHis-Ub (500 µM)........................20 µl
• 10X 6xHis-Ub(K0) (500 µM)................20 µl • 10X 6xHis-Ub(K6R) (500 µM).............20 µl
• 10X 6xHis-Ub(K11R) (500 µM)...........20 µl • 10X 6xHis-Ub(K27R) (500 µM)...........20 µl
• 10X 6xHis-Ub(K29R) (500 µM)...........20 µl • 10X 6xHis-Ub(K33R) (500 µM)...........20 µl
• 10X 6xHis-Ub(K48R) (500 µM)...........20 µl • 10X 6xHis-Ub(K63R) (500 µM)...........20 µl
• 10X ATP (40 mM)................................125 µl • 10X Ubiquitination Buffer.........................1 ml
UBPBio are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capABLe of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.Additional InformationProduct Name:K11-Ub(n≥3)Also Known As:K11-Ub(n≥3)Catalog No.:D3301Size:50 µgMolecular Weight:N/ASpecies:HumanSource:Bacterial recombinantStock:20 mM Tris, 150 mM NaCl, 2 mM βME, 10% GlycerolConcentration:See tube labelQuality Assurance:~95% by SDS-PAGE, see datasheet for gel imageStorage:Store at -80°C; avoid multiple freeze-thaw cyclesPDF Data Sheet:PDF Datasheet， MSDSNCBI RefSeq:N/AImage(s):N/AShipping Method:Dry ice shippingReferences:1. Hershko A, et al. (1980)Proc Natl Acad Sci USA 77(4), 1783 – 1786.2. Pickart CM, (1997) FASEB 11(13), 1055 – 1066.3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.DetailsUb chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s. Description
Ubiquitin Linkage Screening Kit II is designed for two purposes: 1) to identify the ubiquitin lysine residue that is used to link the polyubiquitin chain conjugated on a protein substrate; 2) to determine whether a protein substrate is polyubiquitinated or conjugated with multiple monoubiquitin.
A typical 20 µl reaction contains 100 nM UBE1, 2 µM E2 (not supplied), 50 µM Ub or Ub mutants, 4 mM ATP, 2 µM E3 (not supplied) and 2 µM substrate (no supplied) in the ubiquitination buffer.
Components
• 20X UBE1 (2 µM)..................................50 µl • 10X 6xHis-Ub (500 µM)........................20 µl
• 10X 6xHis-Ub(K0) (500 µM)................20 µl • 10X 6xHis-Ub(K6R) (500 µM).............20 µl
• 10X 6xHis-Ub(K11R) (500 µM)...........20 µl • 10X 6xHis-Ub(K27R) (500 µM)...........20 µl
• 10X 6xHis-Ub(K29R) (500 µM)...........20 µl • 10X 6xHis-Ub(K33R) (500 µM)...........20 µl
• 10X 6xHis-Ub(K48R) (500 µM)...........20 µl • 10X 6xHis-Ub(K63R) (500 µM)...........20 µl
• 10X ATP (40 mM)................................125 µl • 10X Ubiquitination Buffer.........................1 ml
