Additional Information

Product Name:	6xHis-Ube2W
Also Known As:	UBC16; UBC-16; hUBC-16; FLJ11011
Catalog No.:	C4001
Size:	100 µg
Molecular Weight:	18.7 kDa
Species:	Human
Source:	Bacterial recombinant
Stock:	20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration:	See tube label
Quality Assurance:	~90% by SDS-PAGE, see datasheet for gel image
Storage:	Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet:	PDF Datasheet， MSDS
NCBI RefSeq:	NM_001001481
Image(s):	(Click image to enlarge) Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ube2W
Shipping Method:	Dry ice shipping
References:	1. Yin G, et al. (2006) Front Biosci 11, 1500 – 1507. 2. Christensen DE, et al. (2007) Nat Struct Mol Biol 14, 941 – 948. 3. Alpi AF, et al. (2008) Mol Cell 32, 767 – 777. 4. Zhang Y, et al. (2011) Mol Cells 31(2), 113 – 122.

Details

UBPBio are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capABLe of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.Additional InformationProduct Name:K11-Ub(n≥3)Also Known As:K11-Ub(n≥3)Catalog No.:D3301Size:50 µgMolecular Weight:N/ASpecies:HumanSource:Bacterial recombinantStock:20 mM Tris, 150 mM NaCl, 2 mM βME, 10% GlycerolConcentration:See tube labelQuality Assurance:~95% by SDS-PAGE, see datasheet for gel imageStorage:Store at -80°C; avoid multiple freeze-thaw cyclesPDF Data Sheet:PDF Datasheet， MSDSNCBI RefSeq:N/AImage(s):N/AShipping Method:Dry ice shippingReferences:1. Hershko A, et al. (1980)Proc Natl Acad Sci USA 77(4), 1783 – 1786.2. Pickart CM, (1997) FASEB 11(13), 1055 – 1066.3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.DetailsUb chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.