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商品描述
Ube2T is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2T interacts with the E3 ligase called Fanconi anemia complementation group L (FANCL) that is part of a larger complex to regulate DNA damage repair. Once FANCL interacts with Ube2T, the complex is then able to monoubiquitinate FANCD2 that leads to the DNA repair. Ube2T can also interact with the BRCA1/BARD1 complex. PIG50; HSPC150
Additional Information
| Product Name: | 6xHis-Ube2T | ||
|---|---|---|---|
| Also Known As: | PIG50; HSPC150 | ||
| Catalog No.: | C3801 | ||
| Size: | 100 µg | ||
| Molecular Weight: | 22.5 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~90% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_014176 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Zhang QH, et al. (2000) Genome Res 10, 1546 – 1560. 2. Machida YJ, et al. (2006) Mol Cell 23, 589-96. 3. Hao J, et al. (2008) Tumour Biol 29, 195 – 203. 4. Ueki T, et al. (2009) Cancer Res 69(22), 8752 – 8760. |
Details
Ube2T is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2T interacts with the E3 ligase called Fanconi anemia complementation group L (FANCL) that is part of a larger complex to regulate DNA damage repair. Once FANCL interacts with Ube2T, the complex is then able to monoubiquitinate FANCD2 that leads to the DNA repair. Ube2T can also interact with the BRCA1/BARD1 complex.
Images: (Click image to enlarge)
UBPBio are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capABLe of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.Additional InformationProduct Name:K11-Ub(n≥3)Also Known As:K11-Ub(n≥3)Catalog No.:D3301Size:50 µgMolecular Weight:N/ASpecies:HumanSource:Bacterial recombinantStock:20 mM Tris, 150 mM NaCl, 2 mM βME, 10% GlycerolConcentration:See tube labelQuality Assurance:~95% by SDS-PAGE, see datasheet for gel imageStorage:Store at -80°C; avoid multiple freeze-thaw cyclesPDF Data Sheet:PDF Datasheet， MSDSNCBI RefSeq:N/AImage(s):N/AShipping Method:Dry ice shippingReferences:1. Hershko A, et al. (1980)Proc Natl Acad Sci USA 77(4), 1783 – 1786.2. Pickart CM, (1997) FASEB 11(13), 1055 – 1066.3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.DetailsUb chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s. Images: (Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ube2T |
