*COLDSHIPITEM:NEXTDAYDELIVERYREQUIRED

ThisisothermalamplificationmixallowsfluorescencedetectionoftheproductontheGenie®IIplatformbutmayalsobeusedongenericqPCRinstrumentation.Anannealcurvecanthenbegeneratedtoconfirmtheproduct.Thiseliminatestheneedforgelelectrophoresisorturbiditydetectionandallowsforaclosed-tubesystem.

IsothermalMastermixesarefullylicensedforLAMPusebyEikenChemicalCompany.

IsothermalMastermixcanbeusedwithRCA,SMAPandmanyotheramplificationtechnologies.

Figure1ontheleftisafluorometricLAMPcomparisonoftheISO-001mastermixwithGspM2.0,GspMandTinexoDNApolymerases.

LAMPtemplatewas1ngofanAeropyrumpernixK1genomicDNAapprox.5.5x105copies.

TheISO-001mastermixusesanengineeredLFDNApolymeraseisolatedfromGeobacillussp.SSD,GspSSD.TheenzymeshowshighestspeedinafluorescentLAMPreactionandistheenzymeofchoiceforLAMPreactions.

GspSSDLFDNApolymeraseproduceslittlePPiinitsreactionbufferthereforeGspM2.0isamuchbetterchoiceforturbidimetricdetection.

AcomparisonbetweenISO-001,BstandBst2.0inafluorescentdetectionbacterialLAMPreaction,runontheGenie®IIinstrument,isshownintheimage Pro-Lab Diagnostics is a privately held corporation founded in 1974 with its head office in Toronto, Canada and subsidiaries in the U.K. and U.S.A. The company has demonstrated the achievement of quality standards in attaining ISO 13485:2003 (CMDCAS) registration as well as FDA facility registration.Pro-Lab distributes as well as manufactures products within the immunodiagnostics area.Our mission is to provide high quality, cost effective immunodiagnostic products and biological reagents to hospitals, private medical laboratories, veterinary laboratories, and other biological investigative laboratories, while maintaining the quality set by governmental, ISO 13485:2003 (CMDCAS) and other International Standards of Excellence.