Based on the specific, high-affinity interaction of phospho groups with trivalent metal-containing nanoparticles (beads), IMAP is a generic, non-antibody-based platform to assess kinase, phosphatase, and phosphodiesterase activity. An enzyme reaction is performed using fluorescently labeled substrate. Addition of the IMAP Binding System stops the enzyme reaction and initiates binding of the beads to phosphorylated substrates. Binding of the substrate to the beads, which correlates to enzyme activity, can be detected using either FP or TR-FRET as a readout.
Benefits of IMAP Assays
- IMAP provides a complete assay system for screening kinases, phosphatases, and phosphodiesterases
- Because IMAP assays are not antibody-based, they are generic and can be used for any kinase, phosphatase, or phosphodiesterase
- Robust fluorescence signal gives reliable results with good Z factors
- IMAP assays are homogeneous and amenable to miniaturization for greater cost savings
- IMAP assays are available in both FP and TR-FRET detection modes to meet users' screening needs
Available Configurations:
| Configuration | Part Number |
IMAP Evaluation Demo KitOptimized 800 data point IMAP kit which includes calibrators (phosphorylated and un-phosphorylated peptides) to run a calibration curve for either FP or TR-FRET detection | (R8166) |
| IMAP TR-FRET Evaluation Kit with Progressive Binding SystemOptimized 800 data point protocol to run IMAP with the Progressive Binding System; Additional kinase and substrate are required | (R8161) |
| IMAP FP PDE Evaluation Kits | (R8175) |
| IMAP TR-FRET PDE Evaluation Kits Includes optimized protocol, cAMP and cGMP substrates; customer provides their own PDE enzyme | (R8176) |
Additional discounted configurations available for customers performing several high-throughput screens in a year. Please contact your local sales representative or call 1-800-635-5577 for more information.
Molecular Devices的EarlyTox心脏毒性试剂盒EarlyTox™心脏毒性试剂盒提供了一种快速，简单且可靠的基于荧光的方法，可使用干细胞衍生的心肌细胞在生物相关测定中鉴定心脏毒性化合物。利用我们专有的掩盖技术和新颖的钙敏感指示剂，研究人员可以：消除心脏毒性化合物并确定潜在的候选药物在数分钟而不是数小时内测量384个样本最小化染料对节拍特性的非特异性影响减少井间差异并提高数据质量最大的信号动态范围针对三种参考化合物的基准：异丙肾上腺素，索他洛尔和普萘洛尔
