This kit enables the detection of an early apoptosis event based on the measurement of the glutathione content in live mammalian cells using a fluorescence microplate reader. Diminished cellular glutathione (GSH), which is an important anti-oxidant in mammalian cells, occurs at the early stages of mitochondria-associated apoptosis because of GSH efflux. This kit uses monochlorobimane (MCB), a cell permeant dye that has a high affinity for GSH, to detect the cellular GSH level in an apoptotic event. The unreacted dye is almost non-fluorescent. After reaction of the dye with GSH, catalyzed by endogenous glutathione-S-transferase (GST) enzymes, the dye fluoresces blue, with excitation at 394 nm and emission at 490 nm. The intensity of the fluorescence signal generated from the assay reflects the amount of GSH that is present in the cells.

 

 

Available Configurations:

Configuration	Description	Part Number
Explorer Kit	(1) vial of MCB (10 mM, 100 µL)Each kit contains enough material for two (2) 96-well or 384-well microplates.	(R8344)
Bulk Kit	(1) vial of MCB (10 mM, 500 µL)Each kit contains enough material for ten (10) 96-well or 384-well microplates.	(R8345)

 

 

Molecular Devices的EarlyTox心脏毒性试剂盒EarlyTox™心脏毒性试剂盒提供了一种快速，简单且可靠的基于荧光的方法，可使用干细胞衍生的心肌细胞在生物相关测定中鉴定心脏毒性化合物。利用我们专有的掩盖技术和新颖的钙敏感指示剂，研究人员可以：消除心脏毒性化合物并确定潜在的候选药物在数分钟而不是数小时内测量384个样本最小化染料对节拍特性的非特异性影响减少井间差异并提高数据质量最大的信号动态范围针对三种参考化合物的基准：异丙肾上腺素，索他洛尔和普萘洛尔