This kit enables analysis of apoptosis based on caspase-3/7 activity and its consensus target sequence, Asp-Glu-Val-Asp (DEVD). Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. This kit provides a single-step, homogenous assay that is specifically designed for microplate readers. The fluorogenic substrate (Ac-DEVD)₂-R110 contains two DEVD consensus target sequence and is completely hydrolyzed in cell lysate by the enzymes. Hydrolysis of both DEVD peptides releases the green fluorescent dye R110, resulting in a substantial fluorescence increase, with excitation at 490 nm and emission at 520 nm. The assay kit includes the competitive caspase-3/7 inhibitor Ac-DEVD-CHO for use as a negative control. R110 is also provided in the kit for generating a standard curve, which can be used for quantifying caspase-3 activity.  

 

 

Available Configurations:

Configuration	Description	Part Number
Explorer Kit	(1) vial of Rhodamine 110 (80 µM, 1 mL) (2) vials of caspase-3 inhibitor (5 mM, 20 µL) (2) vials of DEVD-R110 (2 mM, 500 µL) (2) vials of lysis buffer (10 mL)Each kit contains enough material for two (2) 96-well or 384-well microplates.	(R8346)

 

 

Molecular Devices的EarlyTox心脏毒性试剂盒EarlyTox™心脏毒性试剂盒提供了一种快速，简单且可靠的基于荧光的方法，可使用干细胞衍生的心肌细胞在生物相关测定中鉴定心脏毒性化合物。利用我们专有的掩盖技术和新颖的钙敏感指示剂，研究人员可以：消除心脏毒性化合物并确定潜在的候选药物在数分钟而不是数小时内测量384个样本最小化染料对节拍特性的非特异性影响减少井间差异并提高数据质量最大的信号动态范围针对三种参考化合物的基准：异丙肾上腺素，索他洛尔和普萘洛尔