AntibodiesOnline/CA12 (Extracellular Domain), (AA 1291) (Active) protein (His tag)/ABIN2003147/20

mayitao  /发布时间:1631813409 /浏览量:153   

ProteinName

CA12

• CAXII
• HsT18816
• CAR12
• 2310047E01Rik
• AI314958
• Ca12
• carbonicanhydraseXII
• carbonicanyhydrase12
• CA12
• Car12

ProteinCharacteristics

ExtracellularDomain,AA1-291

3AA25-301

2AA25-354

1AA1-301

1AA1-354

1AA154-307

1AA161-318

1AA25-124

1AA25-303

1ExtracellularDomain

1TranscriptVariant1

1fulllength

1partial

Origin

Human

7Human

6Mouse(Murine)

2Rat(Rattus)

1Rabbit

Source

HumanCells

8Escherichiacoli(E.coli)

2InsectCells

2Wheatgerm

1HEK-293Cells

1HumanCells

1MammalianCells

1Yeast

ProteinType

Recombinant

BiologicalActivity

Active

Purificationtag/Conjugate

Histag

Application

FunctionalStudies(Func),SDS-PAGE(SDS)

Options

Bulkdiscount

Supplier

SupplierProductNo.

Productdetails ReferencesImagesHandlingApplicationDetailsTargetdetailsbacktotop
Productdetails ShowHide
Characteristics	TherecombinanthumanCA12consistingof278aminoacidsandhasacalculatedmolecularmassof31.6kDa.Asaresultofglycosylation,therecombinantproteinmigratesasanapproximately40-45kDaproteininSDS-PAGEunderreducingconditions. ProteinStructure:ADNAsequenceencodingtheextracellulardomainofhumanCA12(NP_001209.1)(Met1-Gln291)wasexpressedwithaC-terminalpolyhistidinetag. PredictedN-Term:Ala25
Purity	>97 %asdeterminedbySDS-PAGE
EndotoxinLevel	<1.0EUperμgoftheproteinasdeterminedbytheLALmethod
ProductDetails:BiologicalActivityComment	Measuredbyitsesteraseactivity.Thespecificactivityis>40pmoles/min/μg,asmeasuredwith1mM4-Nitrophenylacetateand2.5μgenzymeat400nmin100μLof12.5mMTris,75mMNaCl,pH7.5.

Targetdetails ReferencesImagesHandlingApplicationDetailsbacktotop
Targetdetails ShowHide
Background	Synonyms:CA12,CAXII,FLJ20151,HsT18816
MolecularWeight	31.6kDa,40-45kDa
NCBIAccession	NP_001209
ResearchArea	Cancer

ApplicationDetails ReferencesImagesHandlingTargetdetailsbacktotop
ApplicationDetails ShowHide
ApplicationNotes	Optimalworkingdilutionshouldbedeterminedbytheinvestigator.
Restrictions	ForResearchUseonly

Handling ReferencesImagesApplicationDetailsTargetdetailsbacktotop
Handling ShowHide
Format	Lyophilized
Buffer	LyophilizedfromsterilePBS, pH7.4 Normally5 %-8 %trehalose,mannitoland0.01 %Tween80areaddedasprotectantsbeforelyophilization.
HandlingAdvice	Avoidrepeatedfreeze-thawcycles.Itisrecommendedthattheproteinbealiquotedforoptimalstorage.
Storage	-20°C,-80°C
StorageComment	Storeitundersterileconditionsat-20°Cto-80°C.Itisrecommendedthattheproteinbealiquotedforoptimalstorage.Avoidrepeatedfreeze-thawcycles. Shipping:Ingeneral,recombinantproteinsareprovidedaslyophilizedpowderwhichareshippedatambienttemperature. Bulkpackagesofrecombinantproteinsareprovidedasfrozenliquid.Theyareshippedoutwithblueiceunlesscustomersrequireotherwise.
ExpiryDate	12months

Images ReferencesHandlingApplicationDetailsTargetdetailsbacktotop
Images ShowHide
SupplierImages	CA12(ExtracellularDomain),(AA1-291)(Active)protein(Histag) ×CA12(ExtracellularDomain),(AA1-291)(Active)protein(Histag) CA12(ExtracellularDomain),(AA1-291)(Active)protein(Histag) Close

References ImagesHandlingApplicationDetailsTargetdetailsbacktotop
References ShowHide
Productcitedin:	Lecoeur,Goossens,Vaccher,Bonte,Foulon:"Amultivariateapproachforthedeterminationofisoelectricpointofhumancarbonicanhydraseisoformsbycapillaryisoelectricfocusing."in:Electrophoresis,Vol.32,Issue20,pp.2857-66,2011(PubMed).
Backgroundpublications	Ivanov,Kuzmin,Wei,Pack,Geil,Johnson,Stanbridge,Lerman:"Down-regulationoftransmembranecarbonicanhydrasesinrenalcellcarcinomacelllinesbywild-typevonHippel-Lindautransgenes."in:ProceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica,Vol.95,Issue21,pp.12596-601,1998(PubMed).

================  蚂蚁淘在线  ================

免责声明：本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容

版权声明：未经蚂蚁淘在线授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的，应该授权范围内使用，并注明“来源：蚂蚁淘在线”。违反上述声明者，本网将追究其相关法律责任。