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实验材料酵母菌试剂、试剂盒CM培养基乙酸锂PEGTEDMSO甘油氨苄青霉素仪器、耗材离心机分光光度计摇床培养箱 实验步骤1. 为了转化文库，将约20 ml 含pSH18-34和pBait 的酵母菌EGY48培养液接种