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TwoStepPolymerImmunohistoprobePlusisanextremelysensitiveimmunohistochemistrydetectionsystem.Itisbasedonsignalamplificationbyusingatwo-steppolymerdetectionmethod.Theprimaryantibodyspecifictoanantigenonthetissuesectionisdetectedbyanamplifierforprimaryantibodythenfollowedbypolymerdetector.Theantigensitesarethenintenselyvisualizedwithanappropriatesubstrate/chromogen.ImmunohistoprobePlusdoesnotuseavidinorbiotin,whichcompletelyeliminatednonspecificstainingfromendogenousavidin-biotinactivity.ItiscurrentlythemostsensitivedetectionsystemforIHCapplication.