货号：	11088807001
CAS号：	见产品说明
供应商：	上海妍琦生物
数量：	大量
英文名：	CollagenaseB,100mg
保质期：	见产品批号
保存条件：	见产品说明
规格：	100mg

产品名称ApplicationCollagenasefromC.histolyticumiswidelyusedforthedisaggregationofmanytypesoftissues(e.g.,lung,heart,muscle,bone,ADIposetissue,liver,kidney,cartilage,mammarygland,placentae,bloodvessels,brain,tumors)andforthepreparationofsingle-cellsUSPensionsfortheestablishmentofprimarycellculturesystems.CollagenaseBisrecommendedwhenyieldandviABIlityareimportant.ClostridiumcollagenasefromRochehasbeenusedtopreparecellsfrommanytypesoftissue,suchashepatocytes,adipocytes,pancreaticislets,epithelialcells,musclecells,endothelialcells,etc.However,suitabilityofeachlotoftheenzymefordisruptionofaparticulartissueshouldbedeterminedempirically.GeneraldescriptionCollagenaseBispreparedfromClostridiumhistolyticumculturesbyfiltration,ammoniumsulfateprecipitation,dialysis,andlyophilization.Enzymeactivity:Collagenaseactivity:>0.15U/mg(accordingtoWünsch)(+25°C,4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Argasthesubstrate)Contaminatingenzymeactivities:trypsin,clostripain,andtotalproteolyticactivityCollagenaseBhasanormaltohighcollagenaseactivityandahigherthanaverageclostripainactivity(usually>10U/mg).Inhibitors:Collagenaseinhibitors:EDTA,EGTA,Cys,His,DTT,2-mercaptoethanolCollagenaseisnotinhibitedbyserum.Clostripaininhibitors:TLCKTrypsininhibitors:aprotinin,trypsininhibitor(eggwhite,soybean),serumOtherNotes仅用于生命科学研究。不可用于诊断。PreparationNoteActivator:Ca2+Workingconcentration:0.5to2.5mg/mlStorageconditions(workingsolution):-15to-25°CRocherecommendsreconstitutingonlytheamountoflyophilizateneededforimmediateuse.Thereconstitutedsolutioncanbestoredat-15to-25°Cforuptooneweek.Avoidrepeatedfreezingandthawingsinceactivitydecreasesafterreconstitution.ReconstitutionReconstitutioninanybalancedsaltsolution(e.g.,HBSS)SpecificityCollagenasedegradesnativecollagen.Clostripain,trypsin-likeenzymes,andneutralproteasesdegradeotherproteinsaswell.UnitDefinitionCollagenasefromRocheisassayedinWünschunits(1μmolofproductformedperminuteat+25°CwithWünschsubstrate).Frequently,collagenaseactivitiesaregiveninMandlunits(1μmolleucineliberatedfromcollagenin5hoursat+37°C).Unfortunately,thereisnoconsistentconversionfactorbetweenthetwounitsofactivity,sincetheMandlunitdepends,inpart,ontheconcentrationofcontaminatingproteasesinthecollagenasepreparation,anindefinablevariable.ApurercollagenasepreparationwouldactuallygivealowerspecificactivityinMandlunitsthanacrudepreparation.Clostridiumpreparationstypicallygiveconversionfactorsofapproximately1:1800(e.g.,aparticularlotofClostridiumcollagenasecontainedapproximately0.15WünschU/mgand250MandlU/mg).SpecificationsEnzymeactivity:Collagenaseactivity:>0.15U/mg(accordingtoWünsch)(+25°C,4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Argasthesubstrate)Contaminatingenzymeactivities:trypsin,clostripain,andtotalproteolyticactivityCollagenaseBhasanormaltohighcollagenaseactivityandahigherthanaverageclostripainactivity(usually>10U/mg).Inhibitors:Collagenaseinhibitors:EDTA,EGTA,Cys,His,DTT,2-mercaptoethanolCollagenaseisnotinhibitedbyserum.Clostripaininhibitors:TLCKTrypsininhibitors:aprotinin,trypsininhibitor(eggwhite,soybean),serum性质sterilitynon-sterileformlyophilizedmfr.no.Rochepackagingpkgof100mg(11088807001)pkgof2.5g(11088831001)pkgof500mg(11088815001)concentration0.5-2.5mg/mLoptimumpH6.0-8.0shippedinweticestoragetemp.2-8°C