100XYC(7DropoutAminoAcidLiquid)

1.0garginine0.5gasparticacid0.5gisoleucine0.5gphenylalanine0.5gproline0.5gserine1.0gthreonine0.5gvalineinfinalvolumeof100mlstirwithlowheatuntilintosolutionfiltersterilze

---

10XYNB+tyrosine

17gYNB(w/oaminoacidsandw/oammoniumsulfate)50gammoniumsulfate0.5gtyrosineinafinalvolumeof1Lstirwithlowheatuntilintosolutionfiltersterilze

---

AminoAcidSolutions(100Xstocks,exceptURA=10Xstock)

URA1mg/mlADE1mg/mlLYS10mg/mlMET5mg/mlTRP10mg/mlLEU10mg/mlHIS5mg/mlAutoclavetosterilize,exceptfiltersterilizeTRP

Storeallstocksat4degC,exceptLEU(storeatRT)

---

ExampleRecipeofCompletePlates

20gofAgarin630mldistilledwaterina2LflaskAutoclave20minutesAddthefollowing:

100ml	20%sterileglucose
100ml	10XYNBsolution
10ml	100XYC-7aadropoutsolution
100ml	URAsolution
10ml	ADEsolution
10ml	LYSsolution
10ml	METsolution
10ml	TRPsolution
10ml	LEUsolution
10ml	HISsolution

---

FOAplates

20gofAgarin710mldistilledwaterina2LflaskAutoclave20minutesAddthefollowing:

100ml	20%sterileglucose
100ml	10XYNBsolution
10ml	100XYC-7aadropoutsolution
20ml	URAsolution
10ml	ADEsolution
10ml	LYSsolution
10ml	METsolution
10ml	TRPsolution
10ml	LEUsolution
10ml	HISsolution

Letflaskcoolto50degC,thenadd1.0gof5-FOA.

================  蚂蚁淘在线  ================

免责声明：本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容

版权声明：未经蚂蚁淘在线授权不得转载、摘编或利用其他方式使用上述作品。已经经本网授权使用作品的，应该授权范围内使用，并注明“来源：蚂蚁淘在线”。违反上述声明者，本网将追究其相关法律责任。