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Reto's Buffers & Media Book
蚂蚁淘
/发布时间:1629048602 /浏览量:236
200ml
Glycine75.071.5g
1MMgCl22ml
KOH
50ml
AceticAcid98%(~17M)3ml
H2Oto50ml
Acrylamide/Bisacrylamide
500ml
Acrylamide100g
Bisacrylamide4g
H2Oto500ml
500ml
Glucose4.5g
Tris1.5g
EDTA0.25M20ml
H2Oto450ml
AdjustpH8.0withHClH2O,Autoclave,storeat4°Cto500ml
100ml
Lysozyme0.02g
AlkalineLysisBufferAto100ml
50ml
SDS20%2.5ml
NaOH10M1ml
H2Oto50ml
50ml
KAc14.7g
AceticAcid100%5.75ml
H2Oto50ml
100ml
Amidoblack0.1g
AceticAcid100%7.5ml
Methanol20ml
H2Oto100ml
mlstockfor1litermedium
Adeninesulfate3-Aminophtalhydrazide250mM
10ml
3-Aminophtalhydrazide442mg
H2Oto10ml
50ml
Ammoniummolybdatex4H2O2.1g
H2Oto30ml
HClfuming37%(~12M)16.7ml
H2Oto50ml
Ampicillin20mg/mlpH8.0
15ml
Ampicillin300mg
H2Oto10ml
AdjustpH8.0withNaOHH2O,Filtersterilizeto15ml
Antibiotics
APS
10ml
Ammoniumpersulfate1g
H2O,storeat4°Cto10ml
Ascorbate0.5MpH6.0
50ml
L-Ascorbicacid4.4g
H2Oto22ml
AdjustpH6.0withNaOH1MH2Oto50ml
Asolectin(fattyacidfree)50mg/ml
50ml
Asolectin(acetontreated)2.5g
Diethylether,storeat-20°Cto50ml
ATP200mM
10ml
Adenosine-5"-Triphosphate1.102g
H2Oto5ml
AdjustpH6.5withNaOH1MH2O,storeat-20°Cto10ml
ATPRegeneratingSystem(ARS)40x
1ml
Phospoenolpyruvate(PEP)4.16mg
PyruvateKinase(PK)5U/ml20ml
H2O,storeat4°Cto1ml
10ml
Aprotinin1.5mg/ml3.24ml
PepstatinA1mg/mlinDMSO1.3ml
Leupeptinx1/2H2SO410.5mM37.5ml
4-Aminobenzamidinex2HCl396mg
H2Oto10ml
Bis-Tris1MpH7.0
100ml
Bis-Tris20.9g
H2Oto50ml
AdjustpH7.0withHClH2Oto100ml
Brij/DocpH8.0
100ml
EDTA0.25M0.4ml
Tris1MpH8.01ml
Na-Deoxycholate8%5ml
Brij581g
H2Oto90ml
Dissolveat37°C,adjustpH8.0H2O,Filtersterilizeto100mlBS3BufferpH7.5
1000ml
HEPES23.83g
Dodecylmaltoside0.5g
EDTA0.25M4ml
DTT1.54g
H2Oto900ml
AdjustpH7.5withNaOH10MH2Oto1000ml1.5ml
CdSO4313mg
H2Oto1.5ml
Chloroform
50ml
Chloroform48ml
Isoamylalcohol2ml
15ml
EDTA0.25MpH8.012ml
Tris1MpH8.06ml
H2O3ml
Citrate1MpH5.O
50ml
CitratexH2O10.5g
H2Oto35ml
AdjustpH5.0withNaOH10MH2Oto50mlColonyPCRBuffer10x
15ml
Tris1MpH6.81.875ml
KCl0.629g
H2O,Autoclaveto15ml
CoomassieBlueSolution
1000ml
CoomassieBlue(ServaBlau)2g
Aceticacid75ml
Methanol500ml
H2Oto1000ml
CoumaricAcid40mM
10ml
p-Coumaricacid66mg
H2Oto10ml
CS/PBS
200ml
PBS,20x10ml
Newborncalfserum20ml
H2O,Storefrozen200mlCuSO41M
25ml
CuSO44g
H2Oto25ml
1000ml
H2O1000ml
Diethylpyrocarbonate(DECP)1ml
LetsitatRTfor1h,AutoclaveDestainSolution
1000ml
Aceticacid75ml
Ethanol100ml
H2Oto1000ml
Developer5xw/o
2000ml
Na2CO3200g
H2Oto2000ml
DNALoADIngBuffer6x
10ml
Bromophenolblue25mg
XyleneXyanol25mg
Sucrose4g
H2Oto10ml
Dodecylmaltoside10%
50ml
Dodecylmaltoside5g
H2O,Storeat4°Cto50ml
DTT1M
5ml
Dithiothreitol770mg
H2O,Storeat4°Cto10ml
102ml
Tris-Cl200mMpH8.5100ml
3-Aminophtalhydrazine250mM1ml
Coumaricacid40mM,Filter1ml
ECLSolution2
100ml
H2O100ml
H2O230%60ml
EDTA(Na-Salt)0.25MpH8.0
100ml
EDTA(Na2salt)9.3g
H2Oto70ml
AdjustpH8.0with10MNaOHH2O,Autoclaveto100ml
1.5ml
FeCl3x6H2O406mg
H2Oto1.5ml
Ferrozine100mM
1ml
Ferrozine49.2ml
NaOH1M2ml
H2Oto1ml
Folin50%
50ml
FolinReagens25ml
H2Oto50ml
FPLCSolutionApH7.4
500ml
Tris3.03g
Glycerol87%57.5ml
Dodecylmaltoside10%5ml
H2Oto460ml
AdjustpH7.4with1MH2SO4H2Oto500ml
FPLCSolutionBpH7.4
500ml
Imidazol6.8g
Tris3.03g
Glycerol87%57.5ml
Dodecylmaltoside10%5ml
H2Oto460ml
AdjustpH7.4with1MH2SO4H2Oto500ml
FPLCSolutionCpH7.4
500ml
K2SO417.4g
Tris3.03g
Glycerol87%57.5ml
Dodecylmaltoside10%5ml
H2Oto460ml
AdjustpH7.4with1MH2SO4H2Oto500ml
FrenchPressBuffer(FPB)
500ml
Tris-SO41MpH7.425ml
Glycerol87%57.5ml
H2O,Autoclaveto500ml
1.5ml
Glutathione(GSH)46mg
H2O,Storeat4°Cto1.5ml
Glycerol50%
50ml
Glycerol87%28.75ml
H2O,Autoclaveto50ml
50ml
HCl37%(~12M)4.2ml
H2Oto50ml
HIS-Stock2mg/ml
100ml
L-Histidine200mg
H2O,Autoclaveto100ml
5ml
Isopropyl-b,D-thiogalactoside1.19g
H2O,Filtersterilizeto5ml
15ml
Kanamycin582.6150mg
H2O,Filtersterilizeto15ml
KH2PO40.4M
50ml
KH2PO4136.12722mg
H2Oto50ml
K-Na-Tartrate2%
50ml
K-Na-tartratex4H2O1g
H2Oto50ml
L
10liters
Tris30g
Glycine144g
EDTA(TriplexII)2.9g
SDS10g
H2Oto10liters
LBMedia
1000ml
YeastExtract(BBL)5g
TrypticasePeptone(BBL)10g
NaCl10g
H2O,Autoclaveto1000ml
1000ml
YeastExtract(BBL)5g
TrypitcasePeptone(BBL)10g
NaCl10g
Agar15g
H2O,Autoclaveto1000ml
LBLowSaltLiquid
1000ml
BactoYeastExtract5g
BactoPeptone10g
NaCl5g
H2O,Autoclaveto1000ml
1000ml
BactoYeastExtract5g
BactoPeptone10g
NaCl5g
Agar15g
H2O,Autoclaveto1000ml
LEU-Stock3mg/ml
50ml
L-Leucine150mg
H2O,Filtersterilizeto50ml
LiAc1M
100ml
LiAc-dihydrate10.2g
H2Oto60ml
TE10x10ml
H2O,Autoclaveto100ml
LiCl1M
50ml
LiCl2.12g
H2O,Autoclaveto50ml
LowryComponent
200ml
Na2CO36g
NaOH40800mg
H2Oto200ml
LYS-Stock3mg/ml
50ml
DL-Lysine150mg
H2O,Autoclaveto50ml
M
1000ml
NeopeptoneDifco5g
Bacto-Soytone5g
BeefExtract5g
YeastExtract2.5g
AscorbicAcid0.5g
2-Glycerolphosphatex5H2O,disodiumsaltNote:weused19g/luptoDec.20021g
H2O,Autoclave,ChilltoRTto1000ml
Glucose50%20ml
MgSO41M1ml
50ml
MalachitGreen30mg
H2Oto50ml
MES100mMpH6.4
250ml
MES4.88g
H2Oto200ml
AdjustpH6.4withNaOHH2O,Autoclaveto250ml
15ml
MgSO43.70g
H2Oto15ml
50ml
NaAcx3H2O17g
H2Oto35ml
AdjustpH5.2withAceticAcidca.10ml
H2O,Autoclaveto50ml
Na-Citrate34%
50ml
Na-citratex2H2O17g
H2Oto50ml
NaCl5M
50ml
NaCl14.6g
H2Oto50ml
Na-Deoxycholate8%
30ml
Deoxiycholicacid(Nasalt)4g
H2Oto20ml
AdjustpH8.4with10MNaOHH2O,Filtersterilizeto30ml
NaOH10M
500ml
NaOH200g
H2Oto500ml
NickTranslationBuffer4x
10ml
Tris-Cl1MpH7.22ml
MgSO41M400ml
DTT1M4ml
H2Oto10ml
NiSO41M
1.5ml
NiSO4232mg
H2Oto1.5ml
N-Media
500ml
Na2HPO4x2H2O5.33g
BactoYeastExtract2.5g
BactoPeptone5g
H2O,Autoclaveto490ml
Glucose50%10ml
NTARegenerationBuffer
15ml
Guanidine-HCl(GuHCL)8.6g
AceticAcid98%(~17M)175ml
H2Oto15ml
10ml
Nitrotetrazoliumblue200mg
Dimethylsulfoxide5ml
H2Oto10mlNTP-Mix12.5mMeach
80ml
dATP100mM10ml
dGTP100mM10ml
dCTP100mM10ml
dTTP100mM10ml
H2O40ml
O
500ml
K2Cr2O75g
H3PO40.5ml
H2Oto500ml
P
1000ml
Na2HPO4x2H2O28.5g
NaH2PO4xH2O5.52g
NaCl164g
H2O(pHshouldbe7)to1000ml
PBSaulait
300ml
PBS20x15ml
Defattedpowderedmilk(SanoLait,Coop)6g
Azide,20%stock(tostABIlize;inhibitslactoperoxidase!)0.3ml
H2Oto300ml
PBS-Tween
300ml
PBS20x15ml
Tween20600ml
H2Oto300ml
PEG400050%
100ml
Polyethyleneglycol400050g
H2O,Autoclaveto100ml
50ml
Phenol,distilled50ml
TE50mg
Mercaptoethanol100ml
Phenol(stabilized)
50ml
Phenol(stabilized)50ml
Mercaptoethanol100ml
50ml
Phenol25ml
Chloroform24ml
Isoamylalcohol1ml
PIMProteaseInhibitorMix
20ml
TLCK(Tosyl-Lysinechloromethylketone)369738mg
TPCK(Tosyl-Phenylalaninechloromethylketone)704mg
p-Aminobenzamidin-HCl208416mg
o-Phenanthroline198396mg
PMSF(Phenylmethylsulfonylfluoride)174348mg
DMSO,Store2mlaliquotsat-20°C(keepsupto1yearorlonger)to20ml
PISProteaseInhibitorMix
20ml
TLCK(Tosyl-Lysinechloromethylketone)369738mg
TPCK(Tosyl-Phenylalaninechloromethylketone)704mg
p-Aminobenzamidin-HCl208416mg
PMSF(Phenylmethylsulfonylfluoride)174348mg
DMSO,Store2mlaliquotsat-20°C(keepsupto1yearorlonger)to20ml
PLATE
50ml
PEG400020g
LiAc0.51g
Tris-Cl1MpH7.5500ml
EDTA0.25M200ml
H2O,Autoclaveto50ml
Poly-DL-Lysine
5ml
Poly-DL-Lysine50mg
H2O,storeat-20°Cto5ml
ProteinCrackingBuffer
50ml
Tris1MpH7.51.25ml
NaCl58.40.44g
Dithiothreitol1548mg
H2O,Storeat4°Cto50ml
ProteinRunningGelBuffer1/2
500ml
Tris1MpH8.8375ml
SDS20%5ml
EDTA0.25M8ml
H2Oto500ml
ProteinSampleBuffer3x
20ml
Tris0.1MpH86ml
Glycerol50%12ml
SDS1.5g
DTT(Dithiothreitol)30mg
Bromphenolblue10mg
H2Oto20ml
ProteinStackingGelBuffer1/3
500ml
Tris1MpH6.883ml
SDS20%3.3ml
EDTA0.25M5.3ml
H2Oto500ml
PS5x
1000ml
Na2HPO4x2H2O71.2g
NaH2PO4xH2O13.8g
NaCl29.25g
H2Oto1000ml
PS-Tween
300ml
PBS5x60ml
Tween20600ml
H2Oto300ml
PS-Tweenaulait
15ml
Defattedpowderedmilk(SanoLait,Coop)1.5g
PS-Tweento15ml
Q
500ml
Tris3.03g
H2Oto450ml
AdjustpH7.5with1MH2SO4H2Oto500ml
RusconiMix
10ml
Lysozyme10mg/ml500ml
EDTA0.25M1ml
Tris1MpH7.5250ml
RNaseA10mg/ml100ml
DNALoadingBuffer6x1.5ml
H2Oto10ml
S
1000ml
YeastNitr.Basew/oaa6.7g
Dextrose20g
H2O,Autoclaveto1000ml
1000ml
YeastNitr.Basew/oaa6.7g
Dextrose20g
Agar20g
H2O,Autoclave,cool,pourto1000ml
1000ml
AgNO320g
H2O,storeinabrownbottleto1000ml
250ml
Sorbitol36.43g
H2O,Autoclaveto250ml
SSC20x
1000ml
NaCl175g
Na3-citrate88.2g
H2Oto450ml
AdjustpH7.0with1MHClH2Oto500ml
STET
50ml
Sucrose(Saccharose)4g
TritonX-1002.5ml
EDTA0.25M200ml
Tris-Cl1MpH8.02.5ml
Na-azide20%50ml
H2Oto50ml
Sucrose25%inTrispH8.0
100ml
Sucrose25g
Tris1MpH8.05ml
H2O,Autoclaveto100ml
SulfuricAcid1M
50ml
H2O40ml
H2SO495-97%(~18M)2.8ml
H2Oto50ml
T
100ml
Tris-base1M1ml
EDTA0.25M0.4ml
AdjustpH8.0withHClH2O,Autoclaveto100mlTE10x
100ml
Tris-Cl1MpH8.010ml
EDTA0.25M4ml
H2O,Autoclaveto100ml
500ml
EDTA(Na2salt)18.6g
Tris242g
H2Oto400ml
Aceticacid100%57ml
H2Oto500ml
TES
100ml
Tris1MpH8.05ml
EDTA0.25M2ml
NaCl0.3g
H2O,Autoclaveto100ml
TFB1
500ml
KAc1.473g
RbCl605mg
CaCl2x2H2O735mg
MnCl23.145g
Glycerol87%86ml
H2Oto450ml
AdjustpH5.8with0.2MAcOHH2O,Filtersterilizeto500ml
500ml
MOPS1.047g
RbCl605mg
CaCl2x2H2O5.51g
MnCl23.145g
Glycerol87%86ml
H2Oto450ml
AdjustpH6.5with0.2MKOHH2O,Filtersterilizeto500ml
TricineAnodeBuffer(+)
1000ml
Tris24.23g
H2Oto900ml
AdjustpH8.9withHClH2Oto1000ml
TricineCathodeBuffer(-)
1000ml
Tris12.11g
Tricine17.92g
SDS20%5ml
H2Oto1000mlTricineGelAcrylamide
250ml
Acrylamide120g
Methylene-Bisacrylamide3.75g
H2O,avoidsunlightto250mlTricineGelBuffer
250ml
Tris90.86g
H2Oto200ml
AdjustpH8.45withHClSDS20%3.75ml
H2Oto250mlTris1MpHX
100ml
Tris12.1g
H2Oto80ml
AdjustpHXwithHClH2O,Autoclaveto100ml
100ml
Tris12.1g
H2Oto90ml
AdjustpH7.4withH2SO4H2O,Autoclaveto100ml
TRP-Stock2mg/ml
40ml
L-Tryptophan80mg
H2O,Filtersterilizeto40ml
TSS
50ml
Polyethyleneglycol600060005g
DMSO2.5ml
MgSO41ml
LB,Autoclaveto50ml
U
100ml
Uracil200mg
H2O,Autoclaveto100ml
V
5ml
Na3VO4x5H2O27.4mg
H2O,storeat-20°Cto5ml
W
2000ml
Tris72.7g
Glycine300g
SDS20%4g
H2Oto2000ml
X
10ml
5-Bromo-4-chloro-indoxylphosphate100mg
Dimethylsulfoxideto10ml
Y
50ml
TritonX-1001ml
SDS20%2.5ml
NaCl0.3g
Tris-Cl1MpH8.0500ml
EDTA0.25M200ml
H2Oto50ml
1000ml
BactoYeastExtract10g
BactoPeptone20g
Dextrose20g
H2O,Autoclaveto1000ml
1000ml
BactoYeastExtract10g
BactoPeptone20g
Dextrose20g
Agar20g
H2O,Autoclave,cool,pourto1000ml
1000ml
BactoYeastExtract10g
BactoPeptone20g
Galactose20g
H2O,Autoclaveto1000ml
1000ml
BactoYeastExtract10g
BactoPeptone20g
Galactose20g
Agar20g
H2O,Autoclave,cool,pourto1000ml
Z
1.5ml
ZnSO4x7H2O431mg
H2Oto1.5ml
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