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Reto's Buffers & Media Book
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L M O P Q S T TricineAnodeBuffer(+) TricineCathodeBuffer(-) TricineGelAcrylamide TricineGelBuffer U V W X Y ZCompound Glycine 75.07 1MMgCl2 KOH H2O to200ml 
Compound AceticAcid98%(~17M) H2O
Acrylamide/BisacrylamideCompound Acrylamide Bisacrylamide H2O Compound Glucose Tris EDTA0.25M H2O AdjustpH8.0withHCl H2O,Autoclave,storeat4°C Compound Lysozyme AlkalineLysisBufferA Compound SDS20% NaOH10M H2O Compound KAc AceticAcid100% H2O Compound Amidoblack AceticAcid100% Methanol H2O Constituent Adeninesulfate Uracil L-tryptophan L-histidine-HCl L-arginine-HCl L-methionine L-tyrosine L-leucine L-isoleucine L-lysine-HCl L-phenylalanine L-glutamicacid L-asparticacid L-valine L-threonine L-serine
3-Aminophtalhydrazide250mMCompound 3-Aminophtalhydrazide H2O Compound Ammoniummolybdatex4H2O H2O HClfuming37%(~12M) H2O
Ampicillin20mg/mlpH8.0Compound Ampicillin H2O AdjustpH8.0withNaOH H2O,Filtersterilize
AntibioticsAntibiotics ResistanceMechanism Ampicillin Inhibitscellwallsynthesisbyinhibitingformationofthepeptidoglycancrosslink b-lactamase(286aa)issecretedintotheperiplasmandcleaveslactamantibiotic Chloramphenicol Inhibitsproteinsynthesisbyinteractingwiththe50Sribosomalsubunitandinhibitingthepeptidyltransferasereaction. 216aaproteincytoplasmicproteinwhichacetylateschloramphenicol KanamycinNeomycin Inhibitstranslocationandelicitsmiscodingbybindingtothe30Sribosomalsubunit. 256aaaminoglycosidephosphotransferasephosphorylateskanamycin,neomycin,etc. Tetracycline InhibitsproteinsynthesisbypreventingbindingofaminoacyltRNAtotheribosomeAsite. 428aamembraneproteincatalyzingeffluxoftetracyclin,probablybyacationsymportmechanism.
APSCompound Ammoniumpersulfate H2O,storeat4°C
Ascorbate0.5MpH6.0Compound L-Ascorbicacid H2O AdjustpH6.0withNaOH1M H2O
Asolectin(fattyacidfree)50mg/mlCompound Asolectin(acetontreated) Diethylether,storeat-20°C
ATP200mMCompound Adenosine-5"-Triphosphate H2O AdjustpH6.5withNaOH1M H2O,storeat-20°C
ATPRegeneratingSystem(ARS)40xCompound Phospoenolpyruvate(PEP) PyruvateKinase(PK)5U/ml H2O,storeat4°C Compound Aprotinin1.5mg/ml PepstatinA1mg/mlinDMSO Leupeptinx1/2H2SO410.5mM 4-Aminobenzamidinex2HCl H2O
Bis-Tris1MpH7.0Compound Bis-Tris H2O AdjustpH7.0withHCl H2O
Brij/DocpH8.0Compound EDTA0.25M Tris1MpH8.0 Na-Deoxycholate8% Brij58 H2O Dissolveat37°C,adjustpH8.0 H2O,Filtersterilize to100ml
BS3BufferpH7.5Compound HEPES Dodecylmaltoside EDTA0.25M DTT H2O AdjustpH7.5withNaOH10M H2O to1000ml Compound CdSO4 H2O
ChloroformCompound Chloroform Isoamylalcohol Compound EDTA0.25MpH8.0 Tris1MpH8.0 H2O
Citrate1MpH5.OCompound CitratexH2O H2O AdjustpH5.0withNaOH10M H2O to50ml
ColonyPCRBuffer10xCompound Tris1MpH6.8 KCl H2O,Autoclave
CoomassieBlueSolutionCompound CoomassieBlue(ServaBlau) Aceticacid Methanol H2O
CoumaricAcid40mMCompound p-Coumaricacid H2O
CS/PBSCompound PBS,20x Newborncalfserum H2O,Storefrozen 200ml
CuSO41MCompound CuSO4 H2O Compound H2O Diethylpyrocarbonate(DECP) LetsitatRTfor1h,Autoclave
DestainSolutionCompound Aceticacid Ethanol H2O
Developer5xw/oCompound Na2CO3 H2O Product/Reagent Volume Visking20/32tubing 2%NaHCO3 10mMEDTA 10mMEDTA H2O
DNALoADIngBuffer6xCompound Bromophenolblue XyleneXyanol Sucrose H2O
Dodecylmaltoside10%Compound Dodecylmaltoside H2O,Storeat4°C
DTT1MCompound Dithiothreitol H2O,Storeat4°C Compound Tris-Cl200mMpH8.5 3-Aminophtalhydrazine250mM Coumaricacid40mM,Filter
ECLSolution2Compound H2O H2O230%
EDTA(Na-Salt)0.25MpH8.0Compound EDTA(Na2salt) H2O AdjustpH8.0with10MNaOH H2O,Autoclave Compound FeCl3x6H2O H2O
Ferrozine100mMCompound Ferrozine NaOH1M H2O
Folin50%Compound FolinReagens H2O
FPLCSolutionApH7.4Compound Tris Glycerol87% Dodecylmaltoside10% H2O AdjustpH7.4with1MH2SO4 H2O
FPLCSolutionBpH7.4Compound Imidazol Tris Glycerol87% Dodecylmaltoside10% H2O AdjustpH7.4with1MH2SO4 H2O
FPLCSolutionCpH7.4Compound K2SO4 Tris Glycerol87% Dodecylmaltoside10% H2O AdjustpH7.4with1MH2SO4 H2O
FrenchPressBuffer(FPB)Compound Tris-SO41MpH7.4 Glycerol87% H2O,Autoclave Compound Glutathione(GSH) H2O,Storeat4°C
Glycerol50%Compound Glycerol87% H2O,Autoclave Compound HCl37%(~12M) H2O
HIS-Stock2mg/mlCompound L-Histidine H2O,Autoclave Compound Isopropyl-b,D-thiogalactoside H2O,Filtersterilize Compound Kanamycin 582.6 H2O,Filtersterilize
KH2PO40.4MCompound KH2PO4 136.1 H2O
K-Na-Tartrate2%Compound K-Na-tartratex4H2O H2O Compound Tris Glycine EDTA(TriplexII) SDS H2O
LBMediaCompound YeastExtract(BBL) TrypticasePeptone(BBL) NaCl H2O,Autoclave Compound YeastExtract(BBL) TrypitcasePeptone(BBL) NaCl Agar H2O,Autoclave
LBLowSaltLiquidCompound BactoYeastExtract BactoPeptone NaCl H2O,Autoclave Compound BactoYeastExtract BactoPeptone NaCl Agar H2O,Autoclave
LEU-Stock3mg/mlCompound L-Leucine H2O,Filtersterilize
LiAc1MCompound LiAc-dihydrate H2O TE10x H2O,Autoclave
LiCl1MCompound LiCl H2O,Autoclave
LowryComponentCompound Na2CO3 NaOH 40 H2O
LYS-Stock3mg/mlCompound DL-Lysine H2O,Autoclave Compound NeopeptoneDifco Bacto-Soytone BeefExtract YeastExtract AscorbicAcid 2-Glycerolphosphatex5H2O,disodiumsaltNote:weused19g/luptoDec.2002 H2O,Autoclave,ChilltoRT Glucose50% MgSO41M Compound MalachitGreen H2O
MES100mMpH6.4Compound MES H2O AdjustpH6.4withNaOH H2O,Autoclave Compound MgSO4 H2O Compound MW Finalconcentration 1000ml TrypticasePeptone(BBL) 1% 10g BeefExtract(Difco) 0.3% 3g YeastExtract 0.5% 5g K2HPO4 174 11.5mM 2g NH4Acetate 77 39mM 3g tri-Na-Citratex2H2O 294 10.2mM 3g MgSO41M 246 0.8mM 0.8ml MnSO40.4M 0.4mM 1ml Tween80 0.1% 1ml AdjustpH6.3with5NHCl H2O,Autoclave to1000ml Glucose50%(orLactose=MRSL) 1% 20ml Compound NaAcx3H2O H2O AdjustpH5.2withAceticAcid H2O,Autoclave
Na-Citrate34%Compound Na-citratex2H2O H2O
NaCl5MCompound NaCl H2O
Na-Deoxycholate8%Compound Deoxiycholicacid(Nasalt) H2O AdjustpH8.4with10MNaOH H2O,Filtersterilize
NaOH10MCompound NaOH H2O
NickTranslationBuffer4xCompound Tris-Cl1MpH7.2 MgSO41M DTT1M H2O
NiSO41MCompound NiSO4 H2O
N-MediaCompound Na2HPO4x2H2O BactoYeastExtract BactoPeptone H2O,Autoclave Glucose50%
NTARegenerationBufferCompound Guanidine-HCl(GuHCL) AceticAcid98%(~17M) H2O Compound Nitrotetrazoliumblue Dimethylsulfoxide H2O to10ml
NTP-Mix12.5mMeachCompound dATP100mM dGTP100mM dCTP100mM dTTP100mM H2O Compound K2Cr2O7 H3PO4 H2O Compound Na2HPO4x2H2O NaH2PO4xH2O NaCl H2O(pHshouldbe7)
PBSaulaitCompound PBS20x Defattedpowderedmilk(SanoLait,Coop) Azide,20%stock(tostABIlize;inhibitslactoperoxidase!) H2O
PBS-TweenCompound PBS20x Tween20 H2O
PEG400050%Compound Polyethyleneglycol4000 H2O,Autoclave Compound Phenol,distilled TE ~20ml 8-Hydroxyquinoline Mercaptoethanol
Phenol(stabilized)Compound Phenol(stabilized) Mercaptoethanol Compound Phenol Chloroform Isoamylalcohol
PIMProteaseInhibitorMixCompound TLCK(Tosyl-Lysinechloromethylketone) 369 TPCK(Tosyl-Phenylalaninechloromethylketone) p-Aminobenzamidin-HCl 208 o-Phenanthroline 198 PMSF(Phenylmethylsulfonylfluoride) 174 DMSO,Store2mlaliquotsat-20°C(keepsupto1yearorlonger)
PISProteaseInhibitorMixCompound TLCK(Tosyl-Lysinechloromethylketone) 369 TPCK(Tosyl-Phenylalaninechloromethylketone) p-Aminobenzamidin-HCl 208 PMSF(Phenylmethylsulfonylfluoride) 174 DMSO,Store2mlaliquotsat-20°C(keepsupto1yearorlonger)
PLATECompound PEG4000 LiAc Tris-Cl1MpH7.5 EDTA0.25M H2O,Autoclave
Poly-DL-LysineCompound Poly-DL-Lysine H2O,storeat-20°C
ProteinCrackingBufferCompound Tris1MpH7.5 NaCl 58.4 Dithiothreitol 154 H2O,Storeat4°C
ProteinRunningGelBuffer1/2Compound Tris1MpH8.8 SDS20% EDTA0.25M H2O
ProteinSampleBuffer3xCompound Tris0.1MpH8 Glycerol50% SDS DTT(Dithiothreitol) Bromphenolblue H2O
ProteinStackingGelBuffer1/3Compound Tris1MpH6.8 SDS20% EDTA0.25M H2O
PS5xCompound Na2HPO4x2H2O NaH2PO4xH2O NaCl H2O
PS-TweenCompound PBS5x Tween20 H2O
PS-TweenaulaitCompound Defattedpowderedmilk(SanoLait,Coop) PS-Tween Compound Tris H2O AdjustpH7.5with1MH2SO4 H2O
RusconiMixCompound Lysozyme10mg/ml EDTA0.25M Tris1MpH7.5 RNaseA10mg/ml DNALoadingBuffer6x H2O Compound YeastNitr.Basew/oaa Dextrose H2O,Autoclave Compound YeastNitr.Basew/oaa Dextrose Agar H2O,Autoclave,cool,pour Compound AgNO3 H2O,storeinabrownbottle Compound Sorbitol H2O,Autoclave
SSC20xCompound NaCl Na3-citrate H2O AdjustpH7.0with1MHCl H2O
STETCompound Sucrose(Saccharose) TritonX-100 EDTA0.25M Tris-Cl1MpH8.0 Na-azide20% H2O
Sucrose25%inTrispH8.0Compound Sucrose Tris1MpH8.0 H2O,Autoclave
SulfuricAcid1MCompound H2O H2SO495-97%(~18M) H2O Compound Tris-base1M EDTA0.25M AdjustpH8.0withHCl H2O,Autoclave to100ml
TE10xCompound Tris-Cl1MpH8.0 EDTA0.25M H2O,Autoclave Compound EDTA(Na2salt) Tris H2O Aceticacid100% H2O
TESCompound Tris1MpH8.0 EDTA0.25M NaCl H2O,Autoclave
TFB1Compound KAc RbCl CaCl2x2H2O MnCl2 Glycerol87% H2O AdjustpH5.8with0.2MAcOH H2O,Filtersterilize Compound MOPS RbCl CaCl2x2H2O MnCl2 Glycerol87% H2O AdjustpH6.5with0.2MKOH H2O,Filtersterilize Compound Tris H2O AdjustpH8.9withHCl H2O Compound Tris Tricine SDS20% H2O to1000ml Compound Acrylamide Methylene-Bisacrylamide H2O,avoidsunlight to250ml Compound Tris H2O AdjustpH8.45withHCl SDS20% H2O to250ml
Tris1MpHXCompound Tris H2O AdjustpHXwithHCl H2O,Autoclave Compound Tris H2O AdjustpH7.4withH2SO4 H2O,Autoclave
TRP-Stock2mg/mlCompound L-Tryptophan H2O,Filtersterilize
TSSCompound Polyethyleneglycol6000 6000 DMSO MgSO4 LB,Autoclave Compound Uracil H2O,Autoclave Compound Na3VO4x5H2O H2O,storeat-20°C Compound Tris Glycine SDS20% H2O Compound 5-Bromo-4-chloro-indoxylphosphate Dimethylsulfoxide Compound TritonX-100 SDS20% NaCl Tris-Cl1MpH8.0 EDTA0.25M H2O Compound BactoYeastExtract BactoPeptone Dextrose H2O,Autoclave Compound BactoYeastExtract BactoPeptone Dextrose Agar H2O,Autoclave,cool,pour Compound BactoYeastExtract BactoPeptone Galactose H2O,Autoclave Compound BactoYeastExtract BactoPeptone Galactose Agar H2O,Autoclave,cool,pour Compound ZnSO4x7H2O H2O
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