众所周知，Q32850/Q32851/Q32852/Q32853/q32854/Q32855/Q32856都是Thermo公司旗下Invitrogen品牌专用于qubit荧光计的配套试剂耗材

最近

Thermo qubit 3.0停产下架，qubit 4顺势推出

那么Q32850/Q32851/Q32852/Q32853/q32854/Q32855/Q32856的区别是什么呢？

请听蚂蚁淘一一分解

其中Q32851和q32854是同一个产品，不同的只是包装规格

Q32851是100assays/q32854是 500assays

描述

The qubit dsDNAHS(HighSensitivity)AssayKitisdesignedspecificallyforusewiththe qubit Fluorometer.Theassayishighlyselectivefordouble-strandedDNA(dsDNA)overRNAandisdesignedtobeaccurateforinitialsampleconcentrationsfrom10pg/µLto100ng/µL.Thekitprovidesconcentratedassayreagent,dilutionbuffer,andpre-dilutedDNAstandards.Simplydilutethereagentusingthebufferprovided,addyoursample(anyvolumebetween1µland20µlisacceptable),andreadtheconcentrationusingthe qubit Fluorometer.Commoncontaminants,suchassalts,freenucleotides,solvents,detergents,orproteinarewelltoleratedintheassay.

其中Q32852和Q32855是同一个产品，不同的只是包装规格

Q32852是100assays/Q32855是 500assays

描述

The qubit RNAHSAssayKit,whenusedwiththe qubit Fluorometer,providesanaccurateandselectivemethodforthequantitationoflow-abundanceRNAsamples.TheassayishighlyselectiveforRNAandwillnotquantitateDNA,protein,orfreenucleotides.Commoncontaminants,suchassalts,freenucleotides,solvents,detergents,orprotein,arewell-toleratedintheassay.TheassaykitisdesignedtobeaccurateforRNAsampleconcentrationsbetween250pg/µLand100ng/µL.Thekitprovidesconcentratedassayreagent,dilutionbuffer,andpre-dilutedRNAstandards.Simplydilutethereagentusingthebufferprovided,addyoursample(anyvolumebetween1µLand20µLisacceptable),andreadtheconcentrationusingthe qubit Fluorometer.

其中Q32850和Q32853是同一个产品，不同的只是包装规格

Q32850是100assays/Q32853是 500assays

描述

The qubit dsDNABRAssayKitisdesignedspecificallyforusewiththe qubit Fluorometer.Theassayishighlyselectivefordouble-strandedDNA(dsDNA)overRNAandisdesignedtobeaccurateforinitialsampleconcentrationfrom100pg/µl–1,000ng/µl.Thekitprovidesconcentratedassayreagent,dilutionbuffer,andpre-dilutedDNAstandards.Simplydilutethereagentusingthebufferprovided,addyoursample(anyvolumebetween1µland20µlisacceptable),andreadtheconcentrationusingthe qubit Fluorometer.Commoncontaminants,suchassalts,freenucleotides,solvents,detergents,orproteinarewelltoleratedintheassay.

Q32856是Qubit分析管

描述

qubit assaytubesare500µLthin-walledpolypropylenetubesforusewiththe qubit Fluorometer.500tubesperpackage.

除此之外还有Q33230/Q33211/Q33221/Q33223

1、Q33230/Q33231

前者规格：100T，后者规格：500T

 Qubit 1X dsDNA HS Assay Kit 

描述

TheQubit1XdsDNAHS(High-Sensitivity)AssayKitisdesignedspecificallyforusewiththeQubitFluorometer.Theassayishighlyselectivefordouble-strandedDNA(dsDNA)overRNAandisdesignedtobeaccurateforinitialsampleconcentrationsfrom10pg/µLto100ng/µL.The"1X"dsDNAassaykitprovidesreagentandbufferinaformulationthatisstableasaready-to-usesolutionforuptooneyearafterpreparation.Simplyaddyoursample(anyvolumebetween1µLand20µLisacceptable)andreadtheconcentrationusingaQubit2.0,3,or4fluorometer.Commoncontaminantssuchassalts,freenucleotides,solvents,detergents,andproteinarewell-toleratedintheassay.

Notes:

1.TheQubit1XdsDNAHSAssayworksonQubit2.0,3,and4fluorometers.ItwillnotworkontheoriginalQubit1.0Fluorometer.DownloadtheMyQubit.qbtfilefortheQubit2.0or3fluorometer.

2.500-mLthin-walledPCRtubes(Cat.No.Q32856)arerequiredbutnotincluded.

2、Q33211/Q33212

前者规格：100T，后者规格：500T

Qubit™ProteinAssayKit

描述

TheQubitProteinAssayKitisdesignedspecificallyforusewiththeQubitFluorometer.Usingbetween1and20µlofyoursample,thisassaycanquantitatesamplesrangingfrom12.5µg⁄mlto5mg⁄mlandexhibitslowproteintoproteinvariation.Theassayishighlyselectiveforproteinsandisdesignedtobeaccurateinthepresenceofreducingreagents,butnotinthepresenceofalargeamountofdetergent.Commoncontaminants,suchasreducingreagents(DTT,β-mercaptoethanol),salts,freenucleotides,aminoacids,solvents,orDNA,butnotdetergents,arewelltoleratedintheassay.Slightprotocolmodificationsarerequiredforothercontaminants.Thekitprovidesconcentratedassayreagent,dilutionbuffer,andpre-dilutedBSAstandards.Simplydilutethereagentusingthebufferprovided,addyoursample(anyvolumebetween1µLand20µLisacceptable),andreadtheconcentration.

Whichproducttochooseforfluorometricproteinquantitation?

•For1–20samples:usethisQubitProteinAssayKitwiththeQubitFluorometer

•For20–2000samples:usetheQuant-iTProteinAssayKitwithmicroplatereader

Notes:

1.AllQubitassaykitscanbeusedwiththeQubit1.0,Qubit2.0,Qubit3,andQubit4fluorometers.

2.500µLthin-walledPCRtubesarerequiredbutnotincluded.

3、Q33221/Q33222

前者规格：100T，后者规格：500T

Qubit™RNAIQAssayKit

描述

TheQubitRNAIQAssayKitprovidesafast,simplemethodtocheckwhetheranRNAsamplehasdegradedusingtheQubit4Fluorometer(required).Theassayutilizestwouniquedyes:onethatbindstolarge,intactand/orhighlystructuredRNA(mRNA,tRNA,rRNA),andanotherthatselectivelybindstosmalland/or,degradedRNA.TogethertheyenableyoutoquicklyassessthequalityandintegrityofanRNAsample. 

Touse,simplyaddyoursamplestotheRNAIQworkingsolution,thenmeasureontheQubit4Fluorometer.ResultsarepresentedasanRNAIQnumber(RNAIQ#)thatindicatestheRNAsampleintegrityandquality,andalsoasacalculatedpercentoflargeandsmallRNAinthesample.TheRNAIQ#isavaluefrom1to10,similartootherRNAqualityscores,whereasmallnumberindicatesthatthesampleiscomprisedofmainlysmallRNAandalargernumberindicatesthatthesampleconsistsofmainlylargeRNAorRNAwithtertiarystructure.

Solution-basedcomparedtoelectrophoresismethods

TheRNAIQassayresultscorrelatewellwithelectrophoreticmethods.Theprimarydifferencebetweenthesolution-basedRNAIQassayandtraditionalelectrophoresis-basedtechniquesistheamountoftimeittakestoascertainwhetheranRNAsamplehasdegraded.Gelsareconsideredeasytoperform,butstilltaketimetorun.Microfluidic-basedmethodsnotonlytaketimetoobtainresults,butoftenhavelengthyprocedurestopreparethesamples.Gelormicrofluidic-basedelectrophoresisisstillrecommendedtoobtainfragmentsizeinformationorsizedistributionoftheRNAsample.

Notes:

1.TheQubitRNAIQassaywillonlyworkontheQubit4Fluorometer.ItwillnotworkontheQubit,Qubit2.0,orQubit3fluorometers.

2.500µLthin-walledPCRtubes(Cat.No.Q32856)arerequiredbutnotincluded.

4、Q33223/Q33224

前者规格：100T，后者规格：500T

Qubit™RNAXRAssayKit

描述

TheQubitRNAXR(ExtendedRange)AssayKit,whenusedwiththeQubitFluorometer,providesanaccurateandselectivemethodforthequantitationofhigh-abundanceRNAsamples.TheassayishighlyselectiveforRNAanddoesnotquantitateDNA,protein,orfreenucleotides.Commoncontaminants,suchassalts,freenucleotides,solvents,detergents,andprotein,arewell-toleratedintheassay.TheassaykitisdesignedtobeaccurateforinitialRNAsampleconcentrationsfrom1ng/µLto8µg/µL,providinganassayrangefrom20ng–8µg.Thekitprovidesconcentratedassayreagent,dilutionbuffer,andpre-dilutedRNAstandards.Simplydilutethereagentusingthebufferprovided,addthesample(anyvolumebetween1µLand20µLisacceptable),andreadtheconcentrationusingaQubit2.0,3,or4fluorometer.

Notes:

1.TheQubitRNAXRAssayworksonQubit2.0,3,and4fluorometers.ItwillnotworkontheoriginalQubit1.0Fluorometer.DownloadtheMyQubit.qbtfilefortheQubit2.0or3fluorometer.

2.TheQubitRNAXRAssayKitreplacestheQubitRNABR(broad-range)AssayKit.

3.500-mLthin-walledPCRtubes(Cat.No.Q32856)arerequiredbutnotincluded.