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Protein Arginine Methylation
Typical modification sites: RGG box or RXR sequence motifs R-arginine, G-glycine,X-any aminoacid.Enzymes catalysing protein arginine methylation: PRMT1 from rat and the humanhomologue HRMT1L2, human PRMT2 (HRMT1L1), rat and human PRMT3, mouseCARM1 (PRMT4), human JBP1 (PRMT5).Substrates of PRMTs: In mammalian cells hnRNPs contain about 65% of the totalNG,NG-dimethylarginine found in the cell nucleus. Additionally, fun......阅读全文
Biosynthesis of Proline in Bacteria
Among the twenty amino acids encoded by the genetic code, proline is unique in having a cyclic structure with its side chain connected to the amino gr
曹晓风实验室PNAS发布表观遗传研究新成果
来自中国科学院遗传与发育生物学研究所的研究人员证实,拟南芥蛋白精氨酸甲基转移酶3(protein arginine methyltransferase 3,PRMT3)可影响核糖体RNA前体(pre-rRNA)加工,是核糖体生物合成的必要条件。 论文的通讯作者是中科院遗传与发育生物学研究所的曹
MicroRNA Expression Profiling by Bead Array -4
Bead-array-based microRNA detection technology, including the bio-statistic analysis, is currently not well established or widely used and we have app
基因芯片数据的分析方法
研究背景:基因芯片可以通过探针和荧光标记对某个时间点生物体的全部基因表达量进行检测,探针代表的基因荧光强度通过仪器转换成基本数据。这些数据的背后隐藏着很多的生物学意义,这就需要我们通过生物信息学的方法去分析和挖掘。不同实验设计方案产生的海量芯片数据,其分析方法和思路都大同小异,这里分享一个多组实验设
GST融合蛋白的准备
Preparation of Glutathione-S-Transferase (GST) Fusion ProteinsMargret B. Einarson and Elena N. PugachevaFoxx Chase Cancer Center, Philadelphia,
An Introduction to Biocomputing
An Introduction to BiocomputingVersion 2.01October 1996David Steffen, Ph.D.President,Biomedical Computing, Inc.6626 WestchesterHouston, Texas 77
Protein Crystallization
Background:Proteins, like many molecules, can be prompted to form crystals when placed in the appropriate conditions. In order to crystallize a protei
Size and Shape of Protein Molecules-2
The sedimentation coefficient of a protein is a measure of how fast it moves through the gradient. Increasing the mass of the protein will increase it
Purification of Antiserum or Ascites by Protein A/G Chromatography
1、Required Materials and Equipment(1) Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A o
Size and Shape of Protein Molecules-4
Determining the Molecular Weight of a Protein Molecule—CombiningSandRsà la Siegel and MonteWith the completion of multip
Two-hybrid analysis of genetic regulatory networks2
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
蛋白质提取和纯化
蛋白质提取和纯化(主要内容如下)Protein ExtractionProtein PurificationProtein PrecipitationColumn PreparatioinQ A Posted in the Method ForumProtein Extrac
DNA Immunoprecipitation for the Determination of DNA-Binding Specificity
Andrea J. GossettandJason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon
Size and Shape of Protein Molecules-1
Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron MicroscopyAn important part of ch
QTRAP代表文献回顾
生物分子发表的代表性文献 QTRAP:同时具有三重四极杆和线性离子阱性能的独一无二的LC/MS/MS系统 QTRAP系统最早在ASMS 2002上,作为第一台商用的线性离子阱发布,是世界上唯一的线性离子阱和三重四极杆的复合
Lowry – Protein Determination
Lowry – Protein Determination(From Protein Protocols on CD-ROM Humana Press, 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H. Wat
Immunoprecipitation...
实验概要Protein G, a cell wall component produced by group G Streptococcus strains, binds the Fc part of a wide range of immunoglobulins (Ig’s
大规模蛋白质相互作用研究方法进展(四)
表1 蛋白质相互作用分析相关数据库及网站 网站 资源类型 网址 DIP 蛋白质相互作用http: //dip.doe-mbi.uda.edu INTERACT 蛋白质相互作用http: //bioinf.man.ac.uk/interact
Western blotting样品准备
实验概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentratio
超敏C-反应蛋白的研究现状及临床应用(5)
参考文献:1. Morrow DA, Ridker PM. C-reactive protein, inflammation, and coronary risk. Med Clin North Am, 2000,84:149-161.2. Hashimoto H, Kitagawa K, Houg
蛋白质定量
Quantitative Determination of Peptides by Sulfhydryl (-SH) GroupsNew(Contributed by David Van Horn, Dept. of Chemistry, UC Berkeley Greg B
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近红外分析仪在饲料品质分析的应用场景 近红外分析仪在配合饲料整个生产过程中可以设置的质量监控点,如图1所示的1~5。 1)原料进厂时的品质监控 2)生产工艺过程及时监控:在混合工艺、膨化工艺及制粒工艺设置品质监控点 3)成品质量判断图1:配合饲料生产流程图近红外分析仪在饲料品质分
GFP-Trap如何设计成功的IP实验?
借助Chromotek公司GFP-Trap如何设计成功的IP实验?How to plan an immunoprecipitation of your GFP-fusion protein when using the ChromoTek GFP-Trap®PreambleThis document
Crystallization of Kinesin Family Motor Proteins
Motor proteins of several kinesin family groups have now been crystallized: monomeric Kinesin-1 motor domains from human, rat andNeurospora(Kull
一种基于DNA的通用型蛋白检测系统
摘要:基于抗体的蛋白质检测方法,主要有western blot、ELISA、点杂交以及免疫组化等,这些方法被广泛地应用于科研和诊断领域。在蛋白质的免疫检测过程中,样品蛋白首先结合与特异性一抗上,然后再用携带标签(诸如:荧光染料,放射性元素,酶等)的二抗进行检测。然而,为了避免种间内的交叉反应,必
DNA甲基化分析
The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive
Size and Shape of Protein Molecules-3
The Kirkwood/Bloomfield CalculationThe understanding of how protein shape affects hydrodynamics is elegantly extended by an analysis originally develo
使用CO2恒温摇床解决人胚肾 293 (HEK293) 细胞结团问题
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白,如 G 蛋白偶联受体 (GPCR) ,是无法在最常见的生物制药生产宿主,如:中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主,然而 H
Immunoprecipitation... (二)
3. ImmunoprecipitationImmunoprecipitation can be performed using antibodies by different methods. The use of these methods is based on the requi
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