蛋白质、核酸标记和检测 DIG标记及检测试剂盒DIG(地高辛)DNA标记及检测试剂盒DIGDNAlabelingandDetectionKit：RandomprimedDNAlabelingwithdigoxigenin-dUTP,alkali-labileanddetectionofhybridsbyenzymeimmunoassay原理：用DIG-dUTP为底物，随机掺入法进行DNA标记，通过酶联免疫杂交的方法检测。该试剂盒可以标记25次反应，每次标记的DNA量为10ug-20ug，并可做50张10×10cm膜的杂交和检测。应用：非***性DNA标记及检测试剂盒以检测0.1pg的同源DNA，能检测1ug哺乳动物DNA中的单拷贝基因。跟***性标记系统比较，此试剂盒能快速得到实验结果，而且消除了***性同位素的不安全问题。从DNA标记和杂交能见到检测结果，整个实验过程24小时内能完成。这试剂盒的灵敏度与特异性使它适用于所有的杂交技术以替代***性同位素标记和***自显影术。这个标记方法和检测系统适用于DNA印迹转移、菌落杂交、噬斑杂交及原位杂交。产品号产品规格厂商价格109365711093657910Kit：1kit(25labelingreactionsanddetectionof50blots)Roche7447DIGDNALabelingandDetectionKitApplicationRandom-primedlabelingofDNAprobeswithDIG-11-dUTP,alkali-labileandcolordetectionofDIG-labeledhybrids.DIG-labeledprobesareusedforhybridizationinSouthern,northern,anddotblots,aswellasforcolonyandplaquescreening.DIG-labeledhybridsaredetectedwithananti-DIG-alkalinephosphataseconjugateandthesubstratesNBT(nitrobluetetrazoliumsalt)andBCIP(5-bromo-4-chloro-3-indolylphosphate,toluidiniumsalt),whichgivealight-blueprecipitate.ProductDescriptionSensitivityandspecificity:Thestandardlabelingreactionwith1gtemplatewillgiveayieldof260ngDIG-labeledDNAaftera1-hourincubationat37°C,or780ngaftera20-hourincubation.ThesensitivityofDIGdetectiondependsontheconcentrationoftheDIG-labeledprobeinthehybridizationreaction,andthedurationofthecolorreaction.Table1:SensitivityofDIGdetection.Contents1.UnlabeledControlDNA1,20l,100g/ml2.UnlabeledControlDNA2,20l,200g/ml3.DNADilutionBuffer,2x1ml4.DIG-labeledControlDNA,50l,5.2g/ml5.10xHexanucleotideMix,50l6.10xdTPLabelingMixture,50l7.KlenowEnzyme,labelinggrade,25l,2U/l8.Anti-digoxigenin-AP-conjugate,200l,750U/ml9.NBT/BCIPConcentratedStockSolution,10x1ml10.BlockingReagent,2x50g