KitSummary:
•Detectionmethod-Absorbance(500nm)
•Applications-MeasuringfreebiotinorthesubstitutiondegreeofBiotinylation(biotinlabels)ofproteinsandantibodies

Features&Benefits:
•Simple&convenient
•Thiskitcanbeusedtoestimateaslowas60pmolor15ngofbiotininsolution

KitComponents:
•BiotinAssayBuffer
•BiotinAssayReagentA
•BiotinAssayReagentB
•BiotinStandardSolution(10mMinDMSO)
•BiotinylatedBSA(2mg/ml)

Description:
Biotin(VitaminH)isanimportantbiomoleculethathasbeenwidelyusedinBiotinylationreactionstolabelantibodiesorotherproteinsofinterest.Duetoitslowmolecularweight,biotinusuallydoesnotcauseanysignificantchangesinproteinconformationandbiologicalactivity.Biotinylatedproteins/antibodiesarewidelyisolatedandassayedwithstreptavidinpull-downassay,affinitychromatography,ELISAandWesternblottingetc.Biovisionoffersavarietyofready-to-useBiotinylationreagents(Cat.#2346,2345,2347,2322,2326,2323).Itisoftendesiredtodeterminethedegreeofsubstitutionofsuchbiotinylatedbiomolecules.Biovision’sBiotinAssayKitisbasedonthedifferentialbindingofStreptavidintoBiotinandadye,2-(4-hydroxyazobenzene)benzoicacid(HABA).WhenHABAisboundtoStreptavidin,additionoffreebiotinorbiotinylatedbiomoleculesresultsinitsdisplacementinthereaction.Thesechangesaremanifestedintermsofdecreaseintheoverallabsorption(OD500nm)andcanbequantitativelycorrelatedtotheamountofbiotinpresentinsolution.ThekitalsoprovidesBiotinasaStandardandBiotinylatedBSAasapositivecontrol.Thiskitcanbeusedtoestimateaslowas60pmolor15ngofbiotininsolution.

StorageConditions:
-20°C

ShippingConditions:
gelpack

USAGE:ForResearchUseOnly!NotForUseinHumans.