抗绵羊

Agrisera/Donkey antiSheep IgG (H&L), DyLight® 594 conjugated, min. crossreactivity to human or rab

productinformation
Background

Donkeyanti-SheepIgG(H&L),DyLight®594Conjugated,min.cross-reactivitytohumanorrabbitIgG isasecondaryantibodyconjugatedto DyLight®594,whichbindsto all sheep IgG (H&L) inimmunologicalassays. 

DyLight®550hasAmax=593nm,Emax= 618nm.Antibodiesareareaffinitypurifiedusingsolidphase sheepIgG.

DyLight®isaregisteredtrademarkofThermofisherInc.,anditssubsidaries.

Immunogen

PurifiedsheepIgG,wholemolecule

HostDonkey
ClonalityPolyclonal
Clone
PurityAffinitypurifieddonkeyIgG
FormatLyophilized
Quantity1mg
ReconstitutionForreconstitutionadd1.1mlofsterilewater.Letitstand30minutesatroomtemperaturetodissolve.Preparefreshworkingdilutionsdaily.
StorageStorelyophilizedmaterialat2-8°C.Productisstablefor4weeksat2-8°Cafterrehydration.
Forlongtimestorageafterreconstitution,dilutetheantibodysolutionwithglyceroltoafinalconcentrationof50%glycerolandstoreasliquidat-20°C,topreventlossofenzymaticactivity.
Forexample,ifyouhavereconstituted1mgofantibodyin1.1mlofsterilewateradd1.1mlofglycerol.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.
Testedapplications
Relatedproducts

otherdylightanti-sheepsecondaryantibodies

Additionalinformation

Conjugateispresentin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,1%(w/v)BSA,Protease/IgGfree.0.05%(w/v)sodiumazideisaddedaspreservative.

applicationinformation
Recommendeddilution

1:20 -1:2,000formostapplications

Expected|apparentMW
Confirmedreactivity
Predictedreactivity
Notreactivein
Additionalinformation

Basedon immunoelectrophoresis,thisantibodyreactswith: heavy(γ)chainsonsheepIgG lightchainsonallsheepimmunoglobulins.

Noreactivityisobservedto:non-immunoglobulinsheepserumproteinsIgGfromhumanorrabbit.

BSAandmilkhavetobereplacedbyotherblockingreagents,likedonekyserumorcommercialformulationswhicharefreefrombovineIgG.

Selectedreferences

tobeaddedwhenavailable



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