Agrisera/Donkey antiSheep IgG (H&L), DyLight® 650 conjugated, min. crossreactivity to human or rab相关交流-蚂蚁淘在线


productinformation
Background		Donkeyanti-SheepIgG(H&L)-DyLight®650Conjugated,min.cross-reactivitytohumanorrabbitIgGisasecondaryantibodyconjugatedtoDyLight®650,whichbindstoSheepIgG(H&L)inimmunologicalassays. DyLight®650hasAmax=652nm,Emax=672nm.AntibodiesarepurifiedusingsolidphaseSheepIgG DyLight®isaregisteredtrademarkofThermofisherInc.,anditssubsidaries.
Immunogen		PurifiedSheepIgG,wholemolecule
Host		Donkey
Clonality		Polyclonal
Clone
Purity		AffinitypurifieddonkeyIgG
Format		Lyophilized
Quantity		1mg
Reconstitution		Forreconstitutionadd1.1mlofsterilewater.Letitstand30minutesatroomtemperaturetodissolve.Preparefreshworkingdilutionsdaily.
Storage		Storelyophilizedmaterialat2-8°C.Productisstablefor4weeksat2-8°Cafterrehydration. Forlongtimestorageafterreconstitution,dilutetheantibodysolutionwithglyceroltoafinalconcentrationof50%glycerolandstoreasliquidat-20°C,topreventlossofenzymaticactivity. Forexample,ifyouhavereconstituted1mgofantibodyin1.1mlofsterilewateradd1.1mlofglycerol.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.
Testedapplications
Relatedproducts		otherdylightanti-Sheepsecondaryantibodies
Additionalinformation		Conjugateispresentin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,1%(w/v)BSA,Protease/IgGfree.0.05%(w/v)sodiumazideisaddedaspreservative. Basedonimmunoelectrophoresis,thisantibodyreactswith:heavy(γ)chainsonsheepIgGlightchainsonallsheepimmunoglobulins Noreactivityisobservedto:non-immunoglobulinsheepserumproteins. BSAandmilkhavetobereplacedbyotherblockingreagents,likedonekyserumorcommercialformulationswhicharefreefrombovineIgG.

applicationinformation
Recommendeddilution		1:20-1:2,000formostapplications
Expected\|apparentMW
Confirmedreactivity
Predictedreactivity
Notreactivein
Additionalinformation
Selectedreferences		tobeaddedwhenavailable

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