Agrisera/Donkey antiSheep IgG (H&L), HRP conjugated, min. crossreactivity to human, rabbit IgG/AS1相关交流-蚂蚁淘在线


productinformation
Background		Donkeyanti-sheepIgG(H&L),HRPconjugated,isasecondaryantibodywhichbindstosheepIgGsinimmunologicalassays.Antibodiesareadsorbedagainst human,rabbitIgGandaffinitypurifiedusingsolidphasesheepIgG.
Host		Donkey
Clonality		Polyclonal
Clone
Purity		AffinitypurifiedIgG
Format		Lyophilized
Quantity		1mg
Reconstitution		Forreconstitutionadd1.1mlofsterilewater.Letitstand30minutesatroomtemperaturetodissolve.Preparefreshworkingdilutionsdaily.
Storage		Storelyophilizedmaterialat2-8°C. Forlongtimestorageafterreconstitution,dilutetheantibodysolutionwithglyceroltoafinalconcentrationof50%glycerolandstoreasliquidat-20°C,topreventlossofenzymaticactivity. Forexample,ifyouhavereconstituted1mgofantibodyin1.1mlofsterilewateradd1.1mlofglycerol.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.
Testedapplications
Relatedproducts
Additionalinformation		ThisantibodyreactswiththeheavychainsonsheepIgGandwiththelightchainsonallsheepimmunoglobulinsbasedonimmunoelectrophoresis. Minimumcross-reactivityisobservedtonon-immunoglobulinsheepserumproteinsbasedonimmunoelectrophoresis.Minimumcross-reactivityisobservedwithIgGfromhumanorrabbitbasedonimmunoelectrophoresis. Antibodyissuppliedin10mMsodiumphosphate,150mMsodiumchloride,pH7.2,1%(w/v)BSA,Protease/IgGfreeand0.1%(v/v)KathonCGisusedaspreservative.UseofsodiumazidewillinhibitenzymaticactivityofhorserADIshperoxidase. BSAandmilkhavetobereplacedbyotherblockingreagents,likedonekyserumorcommercialformulationswhicharefreefrombovineIgG.

applicationinformation
Recommendeddilution		1:500-1:5000forimmunohistochemistry(IHC),1:10000-1:50000forELISA/westernblot.
Expected\|apparentMW
Confirmedreactivity
Predictedreactivity
Notreactivein
Additionalinformation
Selectedreferences

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