Agrisera/Donkey antiSheep IgG (H&L), ALP conjugated, min. crossreactivity to human, mouse,rabbit I相关交流-蚂蚁淘在线


productinformation
Background		Donkeyanti-sheepIgG(H&L),ALP(alkalinephosphatase)conjugated,isasecondaryantibodywhichbindstosheepIgGsinimmunologicalassays.Antibodiesareadsorbedagainsthuman,mouse,rabbitIgGandareaffinitypurifiedusingsolidphasesheepIgG.
Host		Donkey
Clonality		Polyclonal
Clone
Purity		AffinitypurifiedIgG
Format		Liquid
Quantity		1mg
Reconstitution
Storage		Non-dilutedantibodyisstablefor4yearsat 2-8°C.Forstorageat-20°Cdiluteantibodysolutionwithanequalvolumeofglyceroltoobtainfinalglycerolconcentrationof50%topreventlossofenzymaticactivity.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.
Testedapplications
Relatedproducts		otherALPconjugatedsecondaryantibodiestosheepIgG
Additionalinformation		ThisantibodyreactswiththeheavychainsonsheepIgGandwiththelightchainsonallsheepimmunoglobulinsbasedonimmunoelectrophoresis. Minimumcross-reactivityisobservedtonon-immunoglobulinsheepserumproteinsbasedonimmunoelectrophoresis. Antibodyissuppliedin30mMtriethanolamine,pH7.2,5mMmagnesiumchloride,0.1mMzincchloride,1%(w/v)BSA,Protease/IgGfree,0.05%(w/v)sodiumazideaspreservative. BSAandmilkhavetobereplacedbyotherblockingreagents,likedonekyserumorcommercialformulationswhicharefreefrombovineIgG.

applicationinformation
Recommendeddilution		1:500-1:2000forELISA/westernblot,1:20-1:2000forimmunohistochemistry(IHC),1:50-1:5000forimmunocytochemistry(ICC).
Expected\|apparentMW
Confirmedreactivity
Predictedreactivity
Notreactivein
Additionalinformation
Selectedreferences

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