Agrisera/Rabbit antiChicken IgY (H&L), HRP conjugated free sample/AS10 1489free/相关交流-蚂蚁淘在线


productinformation
Background		Rabbitanti-chickenIgG/Y(H&L)isasecondaryantibodyconjugatedtoHRP,whichbindstoinimmunologicalassays.AntibodyisaffinitypurifiedusingsolidphasechickenIgG/Y.
Immunogen		PurifiedChickenIgG/Y(H&L)
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		AffinitypurifiedRabbitIgG
Format
Quantity		10 µl(0.91mg/ml)
Reconstitution
Storage		Storelyophilizedmaterialat2-8°C.Forstorageat-20°Cafterreconstitutiondiluteantibodysolutionwithanequalvolumeofglyceroltoobtainfinalglycerolconcentrationof50%topreventlossofenzymaticactivity.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.Rehydratewith1.1mlofdeionizedwater.Preparefreshworkingdilutiondaily.
Testedapplications
Relatedproducts		othersecondaryantibodiesagainstchickenIgG/Y(H&L)
Additionalinformation		Purityofthispreparationis>95%basedonSDS-PAGE.Antibodyconcentrationis1.0mg/ml.Antibodyissuppliedin10mMsodiumphosphate,0.15Msodiumchloride,pH7.2.1%(w/v)B,Protease/IgGfree.Contains0.1%(v/v)KathonCGaspreservativeofbacterialgrowth. Basedonimmunoelectrophoresis,thisantibodyreactswith:heavychainsonchickenIgG(IgY),lightchainsonallchickenimmunoglobulins.Basedonimmunoelectrophoresis,noreactivityisobservedto:non-immunoglobulinchickenserumproteins

applicationinformation
Recommendeddilution		Thisconjugateissuitableforallimmunoassayapplications.Theoptimalworkingdilutionshouldbedeterminedbytheinvestigator.Suggestedstartingdilution(s):1:500-1:5000forImmunohistochemistry1:200-1:5000forELISA/Westernblot
Expected\|apparentMW
Confirmedreactivity
Predictedreactivity
Notreactivein
Additionalinformation
Selectedreferences

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