Agrisera/Goat antiChicken IgY (H&L), DyLight® 800 conjugated/AS11 1827/相关交流-蚂蚁淘在线


productinformation
Background		Goatanti-chickenIgY,DyLight®800Conjugateisasecondaryantibodyconjugatedto DyLight®800,whichbindstohenimmunoglobulinsinimmunologicalassays. DyLight®isatrademarkofThermoFisherScientific,Inc.anditssubsidiaries.
Immunogen		purifiedchickenIgY,wholemolecule
Host		Goat
Clonality		Polyclonal
Clone
Purity		AffinitypurifiedgoatIgG
Format		Lyophilized
Quantity		1mg
Reconstitution		Forreconstitutionadd1.1mlofsterilewater.Letitstand30minutesatroomtemperaturetodissolve.Preparefreshworkingdilutionsdaily.
Storage		Storelyophilizedmaterialat2-8°C.Productisstablefor4weeksat2-8°Cafterrehydration. Forlongtimestorageafterreconstitution,dilutetheantibodysolutionwithglyceroltoafinalconcentrationof50%glycerolandstoreasliquidat-20°C,topreventlossofenzymaticactivity. Forexample,ifyouhavereconstituted1mgofantibodyin1.1mlofsterilewateradd1.1mlofglycerol.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.
Testedapplications		immunohistochemistry(IHC),immunocytochemistry(ICC)
Relatedproducts		otheranti-chickenDylightconjugatedsecondaryantibodies
Additionalinformation		Conjugateispresentin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,1%(w/v)BSA,Protease/IgGfree.0.05%(w/v)sodiumazideisaddedaspreservative. DyLight®800 (Ex=777nm;Em=794nm).

applicationinformation
Recommendeddilution		1:20–1:2000(IHC),1:50–1:5000(ICC)
Expected\|apparentMW
Confirmedreactivity		chickenIgYheavyandlightchains(H&L)
Predictedreactivity		chickenIgYheavyandlightchains(H&L)
Notreactivein		noconfirmedexceptionsfrompredictedreactivityknowninthemoment
Additionalinformation		BasedonimmunoelectrophoresisthisantibodyreactswithheavychainsonchickenIgYandlightchainsonallchickenimmunoglobulins. Noreactivityisobservedtonon-immunoglobulinchickenserumproteinsbasedinimmunoelectrophoresis.
Selectedreferences		tobeaddedwhenavailable

Applicationexample

300µg/wellofArabidopsisthalianaproteinfromwildetypeandAGO1-36knockout,AGO1knockdownmutant(1-25)wereextractedbyTCA-acetoneprecipitationfromfloraltissueandsaturatedin8Mureawereseparatedon15%SDS-PAGE(1mmthickgel)andblottedfor1hourto0.2µmnitrocelluloseat100Vusingwettransfersystem.Blotswereblockedwith0.5%coldfishgelatinfor1hratroomtempwithagitation.Blotwasincubatedintheprimaryantibodyatadilutionof1:250foranhouratRTwithagitation.Theblotswerewashedwith3X15minTBS-TTatRTwithagitation.Blotsasincubatedinthesecondaryantibody(DayLight800®,AgriseraAS111827)1:5000dilutionfor30min.atRTwithagitationandwashed1XwithTBSTTfor15min,1XwithTBSTfor15minbeforescanningwiththeODysseyIRDscanner.

AGO2isenrichedinAGO1knockdownmutant,whichagreeswithalreadypublisheddataHarveyetal.(2011),PLOSOne.

CourtesyofDr.BettyChung,UniversityofCambridge,UnitedKingdom

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