抗鸡

Agrisera/Goat antiChicken IgY (H&L), DyLight® 650 conjugated/AS11 1825/

productinformation
Background

Goatanti-chickenIgY,DyLight®650Conjugateisasecondaryantibodyconjugatedto DyLight®650,whichbindstohenimmunoglobulinsinimmunologicalassays. 

DyLight®isatrademarkofThermoFisherScientific,Inc.anditssubsidiaries.

Immunogen

purifiedchickenIgY,wholemolecule

HostGoat
ClonalityPolyclonal
Clone
PurityAffinitypurifiedgoatIgG
FormatLyophilized
Quantity1mg
ReconstitutionForreconstitutionadd1.1mlofsterilewater.Letitstand30minutesatroomtemperaturetodissolve.Preparefreshworkingdilutionsdaily.
StorageStorelyophilizedmaterialat2-8°C.Productisstablefor4weeksat2-8°Cafterrehydration.
Forlongtimestorageafterreconstitution,dilutetheantibodysolutionwithglyceroltoafinalconcentrationof50%glycerolandstoreasliquidat-20°C,topreventlossofenzymaticactivity.
Forexample,ifyouhavereconstituted1mgofantibodyin1.1mlofsterilewateradd1.1mlofglycerol.Suchsolutionwillnotfreezein-20°C.Ifyouareusinga1:5000dilutionpriortodilutingwithglycerol,thenyouwouldneedtousea1:2500dilutionafteraddingglycerol.Prepareworkingdilutionpriortouseandthendiscard.Besuretomixwellbutwithoutfoaming.
Testedapplicationsimmunohistochemistry(IHC),immunocytochemistry(ICC)
Relatedproducts

otheranti-chickenDylightconjugatedsecondaryantibodies

Additionalinformation

Conjugateispresentin10mMSodiumPhosphate,0.15MSodiumChloride,pH7.2,1%(w/v)BSA,Protease/IgGfree.0.05%(w/v)sodiumazideisaddedaspreservative.

DyLight®650 (Ex=652nm;Em=672nm).

applicationinformation
Recommendeddilution

1:20–1:2000(IHC),1:50–1:5000(ICC)

Expected|apparentMW
ConfirmedreactivitychickenIgYheavyandlightchains(H&L)
Predictedreactivity

chickenIgYheavyandlightchains(H&L)

Notreactivein

noconfirmedexceptionsfrompredictedreactivityknowninthemoment

Additionalinformation

BasedonimmunoelectrophoresisthisantibodyreactswithheavychainsonchickenIgYandlightchainsonallchickenimmunoglobulins.

Noreactivityisobservedtonon-immunoglobulinchickenserumproteinsbasedinimmunoelectrophoresis. 

Selectedreferences

tobeaddedwhenavailable



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