微管蛋白β tubulin/Tubulin β单克隆抗体（内参抗体）

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 MouseAnti-Betatubulin(LoadingControl) antibody

 

产品编号	hz-33034M
英文名称	Betatubulin(LoadingControl)
中文名称	微管蛋白βtubulin/Tubulinβ单克隆抗体（内参抗体）
别    名	Beta4tubulin;Beta5tubulin;betaIbtubulin;Beta1tubulin;ClassIbetatubulin;M40;MGC117247;MGC16435;OK/SWcl.56;OK/SWcl.56;TBB5_HUMAN;TUBB1;TUBB2;TUBB5;TUBB;TUBB1;TUBB2;TUBB5;tubulinbeta1chain;Tubulinbeta2chain;tubulinbeta5chain;Tubulinbetachain;TubulinbetaclassI;tubulinbetapolypeptide;Tubulinbeta-5chain.
产品类型	内参抗体
研究领域	细胞骨架  细胞外基质
抗体来源	Mouse
克隆类型	Monoclonal
克 隆 号	7G3
交叉反应	Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, GuineaPig, Monkey, Insect,Yeast
产品应用	WB=1:5000-20000 ELISA=1:5000-10000 IHC-P=1:400-800 IHC-F=1:200-500 ICC=1:100-500 IF=1:200-500 （石蜡切片需做抗原修复） notyettestedinotherapplications.optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
分 子 量	55kDa
细胞定位	细胞浆
性    状	LyophilizedorLiquid
浓    度	1mg/ml
免 疫 原	KLHconjugatedsyntheticpeptidederivedfromhumanBetatubulin:
亚    型	IgG1
纯化方法	affinitypurifiedbyProteinG
储 存 液	0.01MTBS(pH7.4)with1%BSA,0.03%Proclin300and50%Glycerol.
保存条件	Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.
PubMed	PubMed
产品介绍	background:Thisgeneencodesabetatubulinprotein.Thisproteinformsadimerwithalphatubulinandactsasastructuralcomponentofmicrotubules.Mutationsinthisgenecausecorticaldysplasia,complex,withotherbrainmalformations6.Alternativesplicingresultsinmultiplesplicevariants.Therearemultiplepseudogenesforthisgeneonchromosomes1,6,7,8,9,and13.[providedbyRefSeq,Jun2014]Function:Tubulinisthemajorconstituentofmicrotubules.ItbindstwomolesofGTP,oneatanexchangeablesiteonthebetachainandoneatanon-exchangeablesiteonthealphachain.Subunit:Dimerofalphaandbetachains.MayinteractwithRNABP10.InteractswithPIFO.InteractswithMX1.SubcellularLocation:Cytoplasmic,cytoskeleton.TissueSpecificity:Ubiquitouslyexpressedwithhighestlevelsinspleen,thymusandimmaturebrain.Post-translationalmodifications:SomeglutamateresiduesattheC-terminusarepolyglutamylated.Thismodificationoccursexclusivelyonglutamateresiduesandresultsinpolyglutamatechainsonthegamma-carboxylgroup.AlsomonoglycylatedbutnotpolyglycylatedduetotheabsenceoffunctionalTTLL10inhuman.Monoglycylationismainlylimitedtotubulinincorporatedintoaxonemes(ciliaandflagella)whereasglutamylationisprevalentinneuronalcells,centrioles,axonemes,andthemitoticspindle.Bothmodificationscancoexistonthesameproteinonadjacentresidues,andloweringglycylationlevelsincreasespolyglutamylation,andreciprocally.Theprecisefunctionofsuchmodificationsisstillunclearbuttheyregulatetheassemblyanddynamicsofaxonemalmicrotubules(Probable). Similarity:Belongstothetubulinfamily.SWISS:P07437GeneID:203068Databaselinks: EntrezGene:203068Human EntrezGene:22154Mouse EntrezGene:29214Rat Omim:191130Human SwissProt:P07437Human SwissProt:P99024Mouse SwissProt:P69897Rat Unigene:636480Human Unigene:2458Rat   ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.
产品图片	Sample: U87MGCell(Human)Lysateat40ugSW480Cell(Human)Lysateat40ugPrimary:Anti-Betatubulin(bsm-33034M)at1/2000dilutionSecondary:IRDye800CWGoatAnti-MouseIgGat1/20000dilutionPredictedbandsize:55kDObservedbandsize:55kD Paraformaldehyde-fixed,paraffinembedded(Mousebrain);Antigenretrievalbyboilinginsodiumcitratebuffer(pH6.0)for15min;Blockendogenousperoxidaseby3%hydrogenperoxidefor20minutes;Blockingbuffer(normalgoatserum)at37°Cfor30min;Antibodyincubationwith(Betatubulin)MonoclonalAntibody,Unconjugated(bsm-33034M)at1:400overnightat4°C,followedbyoperatingaccordingtoSPKit(Mouse)(sp-0024)instructionsandDABstaining

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