小鼠抗Bcl6单克隆抗体相关交流-蚂蚁淘在线

MouseAnti-BCL-6 antibody

产品编号	hzm-51096M
英文名称	BCL-6
中文名称	小鼠抗Bcl-6单克隆抗体
别名	B-CellCLL/Lymphoma6;ZincFingerProtein51;ZincFingerAndBTBDomain-ContainingProtein27;B-CellLymphoma5Protein;ProteinLAZ-3;ZBTB27;ZNF51;BCL-5;BCL-6;BCL5;LAZ3;Lymphoma-AssociatedZincFingerGeneOnChromosome3;Cys-His2ZincFingerTranscriptionFactor;ZincFingerTranscriptionFactorBCL6S;B-CellLymphoma6ProteinTranscript;B-CellLymphoma6Protein;BCL6A;BCL6_HUMAN;

研究领域	肿瘤免疫学信号转导细胞凋亡转录调节因子
抗体来源	Mouse
克隆类型	Monoclonal
克隆号	6F5
交叉反应	Human,
产品应用	WB=1:500-5000 ELISA=1:500-5000 IF=1:20-200 （石蜡切片需做抗原修复） notyettestedinotherapplications.optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
分子量	78kDa
性状	LyophilizedorLiquid
浓度	1mg/1ml
免疫原	KLHconjugatedsyntheticpeptidederivedfromhumanBCL-6:676-704
亚型	IgG1
纯化方法	affinitypurifiedbyProteinG
储存液	0.01MTBS(pH7.4)with1%BSA,0.03%Proclin300and50%Glycerol.
保存条件	Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.
PubMed	PubMed
产品介绍	background:TheproteinencodedbythisgeneisazincfingertranscriptionfactorandcontainsanN-terminalPOZdomain.Thisproteinactsasasequence-specificrepressoroftranscription,andhasbeenshowntomodulatethetranscriptionofSTAT-dependentIL-4responsesofBcells.ThisproteincaninteractwithavarietyofPOZ-containingproteinsthatfunctionastranscriptioncorepressors.Thisgeneisfoundtobefrequentlytranslocatedandhypermutatedindiffuselarge-celllymphoma(DLCL),andmaybeinvolvedinthepathogenesisofDLCL.Alternativelysplicedtranscriptvariantsencodingdifferentproteinisoformshavebeenfoundforthisgene.[providedbyRefSeq,Aug2015]Function:Transcriptionalrepressormainlyrequiredforgerminalcenter(GC)formationandantibodyaffinitymaturationwhichhasdifferentmechanismsofactionspecifictothelineageandBIOLOGicalfunctions.Formscomplexeswithdifferentcorepressorsandhistonedeacetylasestorepressthetranscriptionalexpressionofdifferentsubsetsoftargetgenes.RepressesitstargetgenesbybindingdirectlytotheDNAsequence5-TTCCTAGAA-3(BCL6-bindingsite)orindirectlybyrepressingthetranscriptionalactivityoftranscriptionfactors.InGCB-cells,repressesgenesthatfunctionindifferentiation,inflammation,apoptosisandcellcyclecontrol,alsoautoregulatesitstranscriptionalexpressionandup-regulates,indirectly,theexpressionofsomegenesimportantforGCreactions,suchasAICDA,throughtherepressionofmicroRNAsexpression,likemiR155.AnimportantfunctionistoallowGCB-cellstoproliferateveryrapidlyinresponsetoT-celldependentantigensandtoleratethephysiologicalDNAbreaksrequiredforimmunglobulinclassswitchrecombinationandsomatichypermutationwithoutinducingap53/TP53-dependentapoptoticresponse.InfollicularhelperCD4(+)T-cells(T(FH)cells),promotestheexpressionofT(FH)-relatedgenesbutinhibitsthedifferentiationofT(H)1,T(H)2andT(H)17cells.AlsorequiredfortheestablishmentandmaintenanceofimmunologicalmemoryforbothT-andB-cells.SuppressesmacrophageproliferationthroughcompetitionwithSTAT5forSTAT-bindingmotifsbindingoncertaintargetgenes,suchasCCL2andCCND2.Inresponsetogenotoxicstress,controlscellcyclearrestinGCB-cellsinbothp53/TP53-dependedentand-independentmanners.Besides,alsocontrolsneurogenesisthroughthealterationofthecompositionofNOTCH-dependenttranscriptionalcomplexesatselectiveNOTCHtargets,suchasHES5,includingtherecruitmentofthedeacetylaseSIRT1andresultinginanepigeneticsilencingleADIngtoneuronaldifferentiation.Subunit:Homodimer.Interacts(viaBTBdomain)withthecorepressorsBCOR,NCOR1andSMRT/NCOR2;theinteractionsaredirect.FormspreferablyternarycomplexeswithBCORandSMRT/NCOR2ontargetgenepromotersbut,onenhancerelements,interactswithSMRT/NCOR2andHDAC3torepressproximalgeneexpression.InteractswithhistonedeacetylasesHDAC2,HDAC5andHDAC9(viathecatalyticdomain).InteractswithZBTB7andBCL6B.InteractswithSCF(FBXO11)complex;theinteractionisindependentofphosphorylationandpromotesubiquitination.Interacts(whenphosphorylated)withPIN1;theinteractionisrequiredforBCL6degradationupongenotoxicstress.InteractswithZBTB17;inhibitsZBTB17transcriptionalactivity.InteractswithCTBP1,autoinhibitsitstranscriptionalexpression.InteractswithNOTCH1NCIDandSIRT1;leadstoaepigeneticrepressionofselectiveNOTCH1-targetgenes.Interacts(norviaBTBdomainneitheracetylated)withtheNuRDcomplexcomponentsCHD4,HDAC1,MBD3andMTA3;theinteractionwithMTA3inhibitsBCL6acetylationandisrequiredforBCL6transpriptionalrepression.SubcellularLocation:NucleusTissueSpecificity:ExpressedingerminalcenterT-andB-cellsandinprimaryimmaturedendriticcells.Post-translationalmodifications:PhosphorylatedbyMAPK1inresponsetoantigenreceptoractivationatSer-333andSer-343.PhosphorylatedbyATMinresponsetogenotoxicstress.Phosphorylationinducesitsdegradationbyubiquitin/proteasomepathway.Polyubiquitinated.PolyubiquitinatedbySCF(FBXO11),leadingtoitsdegradationbytheproteasome.AcetylatedatLys-379byEP300whichinhibitstheinteractionwithNuRDcomplexandthetranscriptionalrepressorfunction.DeacetylatedbyHDAC-andSIR2-dependentpathways.SWISS:P41182GeneID:604Databaselinks: EntrezGene:604Human EntrezGene:12053Mouse EntrezGene:303836Rat Omim:109565Human SwissProt:P41182Human SwissProt:P41183Mouse Unigene:478588Human Unigene:347398Mouse Unigene:20571Rat ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications

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