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小鼠抗Bcl6单克隆抗体
MouseAnti-BCL-6 antibody
产品编号 | hzm-51096M |
英文名称 | BCL-6 |
中文名称 | 小鼠抗Bcl-6单克隆抗体 |
别 名 | B-CellCLL/Lymphoma6;ZincFingerProtein51;ZincFingerAndBTBDomain-ContainingProtein27;B-CellLymphoma5Protein;ProteinLAZ-3;ZBTB27;ZNF51;BCL-5;BCL-6;BCL5;LAZ3;Lymphoma-AssociatedZincFingerGeneOnChromosome3;Cys-His2ZincFingerTranscriptionFactor;ZincFingerTranscriptionFactorBCL6S;B-CellLymphoma6ProteinTranscript;B-CellLymphoma6Protein;BCL6A;BCL6_HUMAN; |
研究领域 | 肿瘤 免疫学 信号转导 细胞凋亡 转录调节因子 |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | 6F5 |
交叉反应 | Human, |
产品应用 | WB=1:500-5000 ELISA=1:500-5000 IF=1:20-200 (石蜡切片需做抗原修复) notyettestedinotherapplications.optimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
分 子 量 | 78kDa |
性 状 | LyophilizedorLiquid |
浓 度 | 1mg/1ml |
免 疫 原 | KLHconjugatedsyntheticpeptidederivedfromhumanBCL-6:676-704 |
亚 型 | IgG1 |
纯化方法 | affinitypurifiedbyProteinG |
储 存 液 | 0.01MTBS(pH7.4)with1%BSA,0.03%Proclin300and50%Glycerol. |
保存条件 | Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C. |
PubMed | PubMed |
产品介绍 | background:TheproteinencodedbythisgeneisazincfingertranscriptionfactorandcontainsanN-terminalPOZdomain.Thisproteinactsasasequence-specificrepressoroftranscription,andhasbeenshowntomodulatethetranscriptionofSTAT-dependentIL-4responsesofBcells.ThisproteincaninteractwithavarietyofPOZ-containingproteinsthatfunctionastranscriptioncorepressors.Thisgeneisfoundtobefrequentlytranslocatedandhypermutatedindiffuselarge-celllymphoma(DLCL),andmaybeinvolvedinthepathogenesisofDLCL.Alternativelysplicedtranscriptvariantsencodingdifferentproteinisoformshavebeenfoundforthisgene.[providedbyRefSeq,Aug2015]Function:Transcriptionalrepressormainlyrequiredforgerminalcenter(GC)formationandantibodyaffinitymaturationwhichhasdifferentmechanismsofactionspecifictothelineageandBIOLOGicalfunctions.Formscomplexeswithdifferentcorepressorsandhistonedeacetylasestorepressthetranscriptionalexpressionofdifferentsubsetsoftargetgenes.RepressesitstargetgenesbybindingdirectlytotheDNAsequence5-TTCCTAGAA-3(BCL6-bindingsite)orindirectlybyrepressingthetranscriptionalactivityoftranscriptionfactors.InGCB-cells,repressesgenesthatfunctionindifferentiation,inflammation,apoptosisandcellcyclecontrol,alsoautoregulatesitstranscriptionalexpressionandup-regulates,indirectly,theexpressionofsomegenesimportantforGCreactions,suchasAICDA,throughtherepressionofmicroRNAsexpression,likemiR155.AnimportantfunctionistoallowGCB-cellstoproliferateveryrapidlyinresponsetoT-celldependentantigensandtoleratethephysiologicalDNAbreaksrequiredforimmunglobulinclassswitchrecombinationandsomatichypermutationwithoutinducingap53/TP53-dependentapoptoticresponse.InfollicularhelperCD4(+)T-cells(T(FH)cells),promotestheexpressionofT(FH)-relatedgenesbutinhibitsthedifferentiationofT(H)1,T(H)2andT(H)17cells.AlsorequiredfortheestablishmentandmaintenanceofimmunologicalmemoryforbothT-andB-cells.SuppressesmacrophageproliferationthroughcompetitionwithSTAT5forSTAT-bindingmotifsbindingoncertaintargetgenes,suchasCCL2andCCND2.Inresponsetogenotoxicstress,controlscellcyclearrestinGCB-cellsinbothp53/TP53-dependedentand-independentmanners.Besides,alsocontrolsneurogenesisthroughthealterationofthecompositionofNOTCH-dependenttranscriptionalcomplexesatselectiveNOTCHtargets,suchasHES5,includingtherecruitmentofthedeacetylaseSIRT1andresultinginanepigeneticsilencingleADIngtoneuronaldifferentiation.Subunit:Homodimer.Interacts(viaBTBdomain)withthecorepressorsBCOR,NCOR1andSMRT/NCOR2;theinteractionsaredirect.FormspreferablyternarycomplexeswithBCORandSMRT/NCOR2ontargetgenepromotersbut,onenhancerelements,interactswithSMRT/NCOR2andHDAC3torepressproximalgeneexpression.InteractswithhistonedeacetylasesHDAC2,HDAC5andHDAC9(viathecatalyticdomain).InteractswithZBTB7andBCL6B.InteractswithSCF(FBXO11)complex;theinteractionisindependentofphosphorylationandpromotesubiquitination.Interacts(whenphosphorylated)withPIN1;theinteractionisrequiredforBCL6degradationupongenotoxicstress.InteractswithZBTB17;inhibitsZBTB17transcriptionalactivity.InteractswithCTBP1,autoinhibitsitstranscriptionalexpression.InteractswithNOTCH1NCIDandSIRT1;leadstoaepigeneticrepressionofselectiveNOTCH1-targetgenes.Interacts(norviaBTBdomainneitheracetylated)withtheNuRDcomplexcomponentsCHD4,HDAC1,MBD3andMTA3;theinteractionwithMTA3inhibitsBCL6acetylationandisrequiredforBCL6transpriptionalrepression.SubcellularLocation:NucleusTissueSpecificity:ExpressedingerminalcenterT-andB-cellsandinprimaryimmaturedendriticcells.Post-translationalmodifications:PhosphorylatedbyMAPK1inresponsetoantigenreceptoractivationatSer-333andSer-343.PhosphorylatedbyATMinresponsetogenotoxicstress.Phosphorylationinducesitsdegradationbyubiquitin/proteasomepathway.Polyubiquitinated.PolyubiquitinatedbySCF(FBXO11),leadingtoitsdegradationbytheproteasome.AcetylatedatLys-379byEP300whichinhibitstheinteractionwithNuRDcomplexandthetranscriptionalrepressorfunction.DeacetylatedbyHDAC-andSIR2-dependentpathways.SWISS:P41182GeneID:604Databaselinks: EntrezGene:604Human EntrezGene:12053Mouse EntrezGene:303836Rat Omim:109565Human SwissProt:P41182Human SwissProt:P41183Mouse Unigene:478588Human Unigene:347398Mouse Unigene:20571Rat ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications |
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