单甲基组蛋白H3(mono methyl K79)单克隆抗体相关交流-蚂蚁淘在线

MouseAnti-HistoneH3(monomethylK79) antibody

产品编号	hz-33099M
英文名称	HistoneH3(monomethylK79)
中文名称	单甲基组蛋白H3(monomethylK79)单克隆抗体
别名	HistoneCluster3,H3;H3HistoneFamily,MemberT;Histone3,H3;H3FT;H3/G;H3/T;H3t;H3.4;HistoneH3.1t;HIST3H3;HGNC:4778;H31T_HUMAN

产品类型	甲基化抗体
研究领域	染色质和核信号表观遗传学
抗体来源	Mouse
克隆类型	Monoclonal
克隆号	7A5
交叉反应	Human, Mouse, Rat,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） notyettestedinotherapplications.optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
分子量	15kDa
细胞定位	细胞核
性状	Liquid
浓度	1mg/ml
免疫原	KLHconjugatedsyntheticpeptidederivedfromhumanHistoneH3(monomethylK79):
亚型	IgG1
纯化方法	affinitypurifiedbyProteinG
储存液	0.01MTBS(pH7.4)with1%BSA,0.03%Proclin300and50%Glycerol.
保存条件	Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.
PubMed	PubMed
产品介绍	background:Histonesarebasicnuclearproteinsthatareresponsibleforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.Thisgeneisintronlessandencodesareplication-dependenthistonethatisamemberofthehistoneH3family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.ThisgeneislocatedseparatelyfromtheotherH3genesthatareinthehistonegeneclusteronchromosome6p22-p21.3.[providedbyRefSeq,Aug2015]Function:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling. Subunit:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.SubcellularLocation:Nucleus;ChromosomeTissueSpecificity:Expressedintesticularcells.ExpressedduringSphase,thenexpressionstronglydecreasesascelldivisionslowsdownduringtheprocessofdifferentiation.Post-translationalmodifications:Acetylationisgenerallylinkedtogeneactivation.AcetylationonLys-10(H3K9ac)impairsmethylationatArg-9(H3R8me2s).AcetylationonLys-19(H3K18ac)andLys-24(H3K24ac)favorsmethylationatArg-18(H3R17me).AcetylationatLys-123(H3K122ac)byEP300/p300playsacentralroleinchromatinstructure:localizesatthesurfaceofthehistoneoctamerandstimulatestranscription,possiblybypromotingnucleosomeinstability(Bysimilarity).CitrullinationatArg-9(H3R8ci)and/orArg-18(H3R17ci)byPADI4impairsmethylationandrepressestranscription.AsymmetricdimethylationatArg-18(H3R17me2a)byCARM1islinkedtogeneactivation.SymmetricdimethylationatArg-9(H3R8me2s)byPRMT5islinkedtogenerepression.AsymmetricdimethylationatArg-3(H3R2me2a)byPRMT6islinkedtogenerepressionandismutuallyexclusivewithH3Lys-5methylation(H3K4me2andH3K4me3).H3R2me2aispresentatthe3ofgenesregardlessoftheirtranscriptionstateandisenrichedoninactivepromoters,whileitisabsentonactivepromoters(Bysimilarity).MethylationatLys-5(H3K4me),Lys-37(H3K36me)andLys-80(H3K79me)arelinkedtogeneactivation.MethylationatLys-5(H3K4me)facilitatessubsequentacetylationofH3andH4.MethylationatLys-80(H3K79me)isassociatedwithDNAdouble-strandbreak(DSB)responsesandisaspecifictargetforTP53BP1.MethylationatLys-10(H3K9me)andLys-28(H3K27me)arelinkedtogenerepression.MethylationatLys-10(H3K9me)isaspecifictargetforHP1proteins(CBX1,CBX3andCBX5)andpreventssubsequentphosphorylationatSer-11(H3S10ph)andacetylationofH3andH4.MethylationatLys-5(H3K4me)andLys-80(H3K79me)requirepreliminarymonoubiquitinationofH2BatLys-120.MethylationatLys-10(H3K9me)andLys-28(H3K27me)areenrichedininactiveXchromosomechromatin.MonomethylationatLys-57(H3K56me1)byEHMT2/G9AinG1phasepromotesinteractionwithPCNAandisrequiredforDNAreplication(Bysimilarity).PhosphorylatedatThr-4(H3T3ph)byGSG2/haspinduringprophaseanddephosphorylatedduringanaphase.PhosphorylationatSer-11(H3S10ph)byAURKBiscrucialforchromosomecondensationandcell-cycleprogressionduringmitosisandmeiosis.InadditionphosphorylationatSer-11(H3S10ph)byRPS6KA4andRPS6KA5isimportantduringinterphasebecauseitenablesthetranscriptionofgenesfollowingexternalstimulation,likemitogens,stress,growthfactorsorUVirradiationandresultintheactivationofgenes,suchasc-fosandc-jun.PhosphorylationatSer-11(H3S10ph),whichislinkedtogeneactivation,preventsmethylationatLys-10(H3K9me)butfacilitatesacetylationofH3andH4.PhosphorylationatSer-11(H3S10ph)byAURKBmediatesthedissociationofHP1proteins(CBX1,CBX3andCBX5)fromheterochromatin.PhosphorylationatSer-11(H3S10ph)isalsoanessentialregulatorymechanismforneoplasticcelltransformation.PhosphorylatedatSer-29(H3S28ph)byMLTKisoform1,RPS6KA5orAURKBduringmitosisoruponultravioletBirradiation.PhosphorylationatThr-7(H3T6ph)byPRKCBisaspecifictagforepigenetictranscriptionalactivationthatpreventsdemethylationofLys-5(H3K4me)byLSD1/KDM1A.Atcentromeres,specificallyphosphorylatedatThr-12(H3T11ph)fromprophasetoearlyanaphase,byDAPK3andPKN1.PhosphorylationatThr-12(H3T11ph)byPKN1isaspecifictagforepigenetictranscriptionalactivationthatpromotesdemethylationofLys-10(H3K9me)byKDM4C/JMJD2C.PhosphorylationatTyr-42(H3Y41ph)byJAK2promotesexclusionofCBX5(HP1alpha)fromchromatin(Bysimilarity).Ubiquitinated.LysinedeaminationatLys-5(H3K4all)toformallysineismediatedbyLOXL2.AllysineformationbyLOXL2onlytakesplaceonH3K4me3andresultsingenerepression.Similarity:BelongstothehistoneH3family.SWISS:P68431GeneID:8290Databaselinks: EntrezGene:8290Human EntrezGene:8350Human EntrezGene:8351Human EntrezGene:8352Human EntrezGene:8353Human EntrezGene:8354Human EntrezGene:8355Human EntrezGene:8356Human EntrezGene:8357Human EntrezGene:8358Human EntrezGene:8968Human EntrezGene:260423Mouse EntrezGene:319148Mouse EntrezGene:319149Mouse EntrezGene:319150Mouse EntrezGene:319151Mouse EntrezGene:319152Mouse EntrezGene:319153Mouse EntrezGene:360198Mouse EntrezGene:97908Mouse EntrezGene:100364501Rat EntrezGene:100365669Rat EntrezGene:291159Rat EntrezGene:314977Rat EntrezGene:364716Rat EntrezGene:679950Rat EntrezGene:679994Rat EntrezGene:680511Rat EntrezGene:680599Rat EntrezGene:682330Rat EntrezGene:691496Rat Omim:601128Human Omim:602810Human Omim:602811Human Omim:602812Human Omim:602813Human Omim:602814Human Omim:602815Human Omim:602816Human Omim:602817Human Omim:602818Human Omim:602819Human SwissProt:P68431Human SwissProt:P84243Human SwissProt:Q16695Human SwissProt:Q6NXT2Human SwissProt:Q71DI3Human SwissProt:P68433Mouse SwissProt:P84228Mouse SwissProt:Q6LED0Rat Unigene:132854Human Unigene:247813Human Unigene:247814Human Unigene:248176Human Unigene:443021Human Unigene:484990Human Unigene:532144Human Unigene:533292Human Unigene:546315Human Unigene:586261Human Unigene:591778Human Unigene:221301Mouse Unigene:261657Mouse Unigene:377874Mouse Unigene:390558Mouse Unigene:397328Mouse Unigene:138090Rat ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.
产品图片	Paraformaldehyde-fixed,paraffinembedded(Mousebrain);Antigenretrievalbyboilinginsodiumcitratebuffer(pH6.0)for15min;Blockendogenousperoxidaseby3%hydrogenperoxidefor20minutes;Blockingbuffer(normalgoatserum)at37°Cfor30min;Antibodyincubationwith(HistoneH3(monomethylK79))MonoclonalAntibody,Unconjugated(bsm-33099M)at1:400overnightat4°C,followedbyoperatingaccordingtoSPKit(Mouse)(sp-0024)instructionsandDABstaining. Paraformaldehyde-fixed,paraffinembedded(Ratbrain);Antigenretrievalbyboilinginsodiumcitratebuffer(pH6.0)for15min;Blockendogenousperoxidaseby3%hydrogenperoxidefor20minutes;Blockingbuffer(normalgoatserum)at37°Cfor30min;Antibodyincubationwith(HistoneH3(monomethylK79))MonoclonalAntibody,Unconjugated(bsm-33099M)at1:400overnightat4°C,followedbyoperatingaccordingtoSPKit(Mouse)(sp-0024)instructionsandDABstaining. Paraformaldehyde-fixed,paraffinembedded(Humancoloncarcinoma);Antigenretrievalbyboilinginsodiumcitratebuffer(pH6.0)for15min;Blockendogenousperoxidaseby3%hydrogenperoxidefor20minutes;Blockingbuffer(normalgoatserum)at37°Cfor30min;Antibodyincubationwith(HistoneH3(monomethylK79))MonoclonalAntibody,Unconjugated(bsm-33099M)at1:400overnightat4°C,followedbyoperatingaccordingtoSPKit(Mouse)(sp-0024)instructionsandDABstaining

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