抗TubulinAlpha鼠单克隆抗体相关交流-蚂蚁淘在线

抗TubulinAlpha鼠单克隆抗体

mayitao /发布时间:1627579802 /浏览量:52

拓然生物经典产品：

Applications

Testedapplications	ELISA,IHC,IF,WB
Citedapplications	WB
Speciesspecificity	Human,Mouse,Rat;otherspeciesnottested.
Citedspecies	Human
PositiveWBdetectedin	Multi-cells,A431cells,HeLa,HeLacells,L02cells,SW1990cells
PositiveIHCdetectedin	Humanlivercancertissue,humantestistissue
PositiveIFdetectedin	Helacells,MCF-7cells
Recommendeddilution	WB:1:500-1:5000IHC:1:50-1:200IF:1:20-1:200

Applicationkey:WB=Westernblotting,IHC=Immunohistochemistry,IF=Immunofluorescence,IP=Immunoprecipitation

Images

Prev12NextViewmorevalidationdata

Westernblotanalysisofalpha-tubulininvarioustissuesandcelllinesusingProteinTechantibody66031-1-Igatadilutionof1:10000.
WesternblotofHelacellwithanti-Tubulin-Alpha(66031-1-Ig)atvariousdilutions.
ImmunofluorescentanalysisofHelacells,usingTUBA1Bantibody66031-1-lgat1:50dilutionandRhodamine-labeledgoatanti-mouseIgG(red).
Immunohistochemicalofparaffin-embeddedhumanlivercancerusing66031-1-Ig(Tubulin-Alphaantibody)atdilutionof1:100(under40xlens)
Immunohistochemicalofparaffin-embeddedhumanlivercancerusing66031-1-Ig(Tubulin-Alphaantibody)atdilutionof1:100(under10xlens)

ProductInformation

Source	Mouse	Purificationmethod	ProteinApurification
Isotype	IgG2b	Storage	PBSwith0.02%sodiumazideand50%glycerolpH7.3.Storeat-20^oC.

ImmunogenInformation

Immunogen	AlphaTubulinfusionproteinag18034	Fullname	Tubulin,alpha1b
Calculatedmolecularweight	50kd	Observedmolecular weight	52kD
GenBankaccession number	BC009314	GeneID(NCBI)	10376
Genesymbol	TUBA1B	Synonyms	AlphaTubulin,Alphatubulinubiquitous,TUBA1B,Tubulinalpha,tubulin,alpha1b

Background

Therearefivetubulinsinhumancells:alpha,beta,gamma,delta,andepsilon.Tubulinsareconservedacrossspecies.Theyformheterodimers,whichmultimerizetoformamicrotubulefilament.Analphaandbetatubulinheterodimeristhebasicstructuralunitofmicrotubules.Theheterodimerdoesnotcomeapart,onceformed.Thealphaandbetatubulins,whichareeachabout55kDaMW,arehomologousbutnotidentical.Alpha,beta,andgammatubulinshaveallbeenusedasloADIngcontrols.Tubulinexpressionmayvaryaccordingtoresistancetoantimicrobialandantimitoticdrugs.ThisantibodyrecognizeTubulinAlphaonly.

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