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1. 把透析袋剪成适当长度（10-20cm）的小段。2. 在大体积的2%(W/V)碳酸氢钠和 1mmol/L EDTA(pH 8.0)中将透析袋煮沸10分钟。3. 用蒸馏水彻底清洗透析袋。4. 放在 1mmol/L EDTA(pH 8.0)