Dialysis(usingdialysistubing)

Molecularpourousmembranetubingisusedfordesalting,proteinandDNAisolation/purification.Itcomesinseveraldiametersandporesizes(formolecularweightconversionsfornucleicacidsseegeneralreference).

ThebrandusedinM.SoaresLabisSpectra/PorproducedbySpectrumMedicalIndustries,Inc.(60916TerminalAnnex,LosAngeles,California90060,Phone:(213)650-2100,(800)634-3300),distributedbyFisher.Severalmolecularweightcut-offsanddiametersareavailable.Spectraportubingcomeseitherwet(readytouse)ordry.

Priortouseofdrymembranes:

1. Wash30minutesat60°Cinalargevolume(1000x)of2%sodiumbicarbonateand1mMEDTA)
2. Rinsetubingthoroughlywithdistilledwater.
3. Coolandstoreat4°Cineither1%formaldehyde,1%sodiumbenzoate,or0.1%sodiumazide,deionizedwater,or100%ethanol.Keepthetubingcompletelysubmersed.Uncleanedmembranescanbestoredatroomtemperature.
4. Donotautoclave.Heatingover80°Cchangesthemembranecharacteristics(droppermeABIlity).
5. TopreventstickingofDNAorchargedproteinstothemembrane,coatwettubinginsideandoutwithasolutionof0.05%BSAin1xTBEbuffer.
6. ThepHrangeformembranesis2-12.

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