Megazyme/Aspartame Assay Kit/KASPTM/50 assays (manual) / 500 assays (microplate) / 500 assays (auto相关交流-蚂蚁淘在线

Megazyme/Aspartame Assay Kit/KASPTM/50 assays (manual) / 500 assays (microplate) / 500 assays (auto

苏州蚂蚁淘 /发布时间:1630519202 /浏览量:36

TheAspartametestkitisasimpleandreliablemethodforthespecificmeasurementandanalysisofAspartameinbeveragesandfoodstuffs.

Suitableformanual,auto-analyserandmicroplateformats.

EnhancementofrecombinantproteinproductioninEscherichiacolibycoproductionofaspartase.

Wang,Z.W.,Chen,Y.&Chao,Y.P.(2006).JournalofBiotechnology,124(2),403-411.

LinktoArticle

ReadAbstract

Ascommonlyrecognized,theexcretionofacetatebytheaerobicgrowthofEscherichiacolionglucoseisamanifestationofimbalancedfluxbetweenglycolysisandthetricarboxylicacid(TCA)cycle.Accordingly,thismayrestricttheproductionofrecombinantproteinsinE.coli,duetothelimitedamountsofprecursormetabolitesproducedinTCAcycle.Toapproachthisissue,anextrasupplyofintermediatemetabolitesinTCAcyclewasmadebyconversionofaspartatetofumarate,areactionmediatedbytheactivityofL-aspartateammonia-lyase(aspartase).Asaresult,intheglucoseminimalmediumcontainingaspartate,theproductionoftworecombinantproteins,β-galactosidaseandgreenfluorescentprotein,intheaspartase-producingstrainwassubstantiallyincreasedby5-foldinassociationwith30–40%morebiomassproduction.Thispreliminarystudyillustratesthegreatpromiseofthisapproachusedtoenhancetheproductionofthesetworecombinantproteins.

UV-methodforthedeterminationofAspartame(andbreakdown
products)infoodstuffs,beveragesandothermaterials

Principle:
            (pH12.5)
(1)Asp-Phe-O-Me→Asp-Phe+MeOH

           (dipeptidaseM)
(2)Asp-Phe+H₂O→L-aspartate+L-phenylalanine

           (glutamate-oxaloacetatetransaminase)
(3)L-Aspartate+2-oxoglutarate→L-glutamate+oxaloacetate

                 (L-malatedehydrogenase)
(4)Oxaloacetate+NADH+H⁺→L-malate+NAD⁺

Kitsize: *50assays(manual)/500(microplate)
/500(auto-analyser)

* Thenumberofmanualtestsperkitcanbedoubledifallvolumesarehalved.
ThiscanbereadilyaccommodatedusingtheMegaQuant^TMWave
Spectrophotometer(D-MQWAVE).

Method:                          Spectrophotometricat340nm
Reactiontime:                 ~5min
Detectionlimit:                0.57mg/L
Applicationexamples:
Softdrinks,artificialsweeteners,candies,mints,chewinggum,dietetic
products,jam,chocolateandothermaterials
Methodrecognition:    Novelmethod

Advantages

Verycosteffective
Allreagentsstablefor>12monthsafterpreparation
Onlyenzymatickitavailable
Measuresaspartameandbreakdownproducts(L-aspartateandaspartameacid)
Veryspecific
Veryrapidreaction
Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing
Standardincluded
Suitableformanual,microplateandauto-analyserformats

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