TransfectionMedium20ml

ExpressionSystems’TransfectionMediumisdesignedtocomplementESF921andESFAFinsectcellculturemediafortheco-transfectionortransfectionstepinbaculovirusvectorproduction.TransfectionMediumisaserum-freeandanimal-freeformulationthatfacilitatesvectorDNAuptakebytheinsecthostcells. 

Description:

ExpressionSystems’TransfectionMediumisdesignedtocomplementESF921andESFAFinsectcellculturemediaforthecotransfectionortransfectionstepinbaculovirusvectorproduction.TransfectionMediumisaserum-freeandanimal-freeformulationthatfacilitatesvectorDNAuptakebytheinsecthostcells.RealizehighertitersbyusingaformulationdesignedtoworkwithESF921andESFAF.

• Serum-free,animal-freeformulationworksseamlesslywithESF921andESFAF
• AppropriateforPEIorlipofectionmediatedtransfection
• OptimizedforusewithExpres²TRTransfectionReagent
• Increasesvirusvectorproductionandtransientexpression
• Availablein20mLor100mLbottles
• Instockforimmediateshipment

 

ExpressionSystems’TransfectionMediumwascomparedtooldertechnologythatusesGrace’smediumfortheperformanceofcotransfectiontoproducebaculovirusvectors.SerumFreeMediumwasincludedasacontrol.ResultingvirustitersfromthecotransfectionwereseverallogshigherusingTransfectionMediumascomparedtoothermedia.TransientexpressionwasalsodeterminedasameasureofDNAuptake.Again,TransfectionMediumresultedinsignificantlybetterresultswiththepercentageofgp64expressingcellsbeingatleasttwo-foldhigherthanothermedia.

Sf9cellsweregrowninSerumFreeMediumandplatedintoindividualwellsofadeepwellblockataconcentrationof2x10⁶cellsper100μlperwell.DNAandalipofectionreagentwereincubatedtogetherin200μloftestmedia(eitherSerumFreeMedium,Grace’sMediumorExpressionSystems’TransfectionMedium)for30minutes.DNAmixturewasaddedtocellsandvolumewasbroughtupto1mlintestmedia.CellsUSPensionwasincubatedforfourhoursat27°Cwhileshaking.Attheendoftheincubation,thevolumeofeachwellwasbroughtupto4mlwithSerumFreeMediumandculturedforfourdays.DeterminationoftheP0(i.e.,productofthecotransfection)infectiousunits(IU)viraltiterofthesupernatantwasperformedusingthegp64flowcytometricmethod.Transientexpressionofgp64wasdeterminedfortheculturedcellsbystainingwithgp64-PE.

Specification:

Mediatype	Serum-free,animal-freeliquid,1X
Shippingcondition	Ambient
Storagecondition	2-8⁰C,protectfromlight
Use-bydate	Oneyearfromdateofmanufacture

RecommendedStorageCondition

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