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ExpressionSystems’popularBestBacLinearizedBaculovirusDNAbackbonehasbeenengineeredtodeliverhighlyefficientrecombinationwithallpolyhedrongenelocusbasedtransfervectors.TheresultingvirusvectorprovidessuperiorstABIlityandpuritycomparedtoothersystems.BestBacisavailablewithadeletionorwild-typeattheV-cath/chiAregionandsoldindividuallyorinkits.
Description:
- UnmatchedproductivityandstabilityforBEVS
- Improvedproductionmethodologycanreduceneedforplaquepurification
- Produceshightitersof5E8to3E9IU/mlofrecombinantbaculovirusinP1stock
- GeneratesP1stockasquicklyasBac-to-Bac®andothersystems
- EachBestBacvialcontainsenoughmaterialforaminimumoffivecotransfections
- SuitableforotherexpressionsystemsincludingBacMamTM
AvailableinTwoVersions
- BestBac1.0iswildtypeatthecathepsinandchitinase(v-cath/chiA)loci,preferredforsomeapplications.
- BestBac2.0isadeletionmutantofBestBac1.0.Thev-cath/chiAdeletionremovesapotentiallydetrimentalproteaseandmayreducecompetitionforresourcesforproteinsynthesisduringlategeneexpression.Thev-cath/chiAdeletioncanimprovetheintegrityofyourexpressedproteinresultinginasignificantincreaseoffunctionalproteinyield.
AlsoAvailableinaCotransfectionKit
Containseverythingyouneedtoperformcotransfectionswithyourtransfervector.Includedinthekitare:
- BestBacDNA
- pVL1393-lacZcontrolvector
- Transfectionmedium
- Expres2TRtransfectionreagent
Specification:
Mediatype | LinearizedBaculovirusDNA |
Shippingcondition | 2–8⁰C |
Storagecondition | 2-8⁰C,protectfromlight |
Use-bydate | Oneyearfromdateofmanufacture |