ExpressionSystemsispleasedtoofferbaculovirustiteringservicestohelpourcustomersmaximizeexpressionandreproducibility.BaculovirustiteringisroutinelysetuponWednesdayswithdatadeliveryThursdayafternoonPST.Samplesshouldbereceivedby11AMWednesdaytobesetupthatweek.
Description:
Accuratetiteringofrecombinantbaculovirusisanimportantstepinproteinproductionoptimization.Ahighthroughput,flowcytometricassayisutilizedtoquantitateinfectiousbaculovirusparticlesproducedininsectcellculture,allowingforaccuratevirustiterdeterminationformultipleconditionsinlessthan24hours.Theassaydetectsthecellsurfaceexpressionofgp64afterashortincubationperiodallowingforasingleroundofinfection.Virusinfectedcellsarethenassessedbyflowcytometricanalysisforgp64expression.
Ship0.5-1mlofvirusstock(aslittleas200µlcanbetiteredwithadvancenotice)
Shipsterilealiquotsin2mlscrewcaptubesorequivalent.Shipovernightoncoldpacks.
Pleaseusetheformonthispageunderthe“TiteringServiceForm”taboratcheckout.
Shipto:
Attention:TiteringService
ExpressionSystems
25372ndStreet
Davis,CA95618
Phone:530-747-2035
Specification:
BaculovirusTiterServiceFAQ
HowmuchvirusdoIneedtoship?
Weprefer1.0mLbutcanruntheassaywith0.5mL.Ifnecessary,wecanuseaslittle200μLbutthatrequiresadvancenotice.
HowdoIneedtoshipmyvirussamples?
Samplesneedtobesterileandshouldbeideallysentincryovialsorsomeothersmallscrewcaptube.Eppendorftubesand15mLconicalsarenotappropriate.(Wehaveexperiencedcrackingof15mLconicaltubesduringshipment.)
ThesampleshouldNOTbefrozenbutshouldbeshippedovernightoncoldpacks.Forcustomersthatwouldliketosubmitmorethan10samples,werequestadvancenotice(theMondaybeforetheyship,forexample,isareasonablenotice).
HowdoIplaceanorder?
Toplaceaphoneorder,completethetiteringserviceformandphoneinpaymentbycalling5307472035.Topaybypurchaseorder,creditcardordebitcard,visittheBaculovirusTiteringServicepage
WheredoIshipmysamples?
Attention:TiteringService
ExpressionSystems
25372ndStreet
Davis,CA95618
Phone:530-747-2035
WhenwillIreceivemyresults?
TiterdataforsamplesreceivedbynoononWedareemailedThursdayafternoonPacificStandardTime.