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ESF921DeltaSeriesFormulationsarebasedonthepopularESF921mediumandareavailableinavarietyofaminoaciddeficientformatsusedforstructuralanalysisandproteinlabeling. Offtheshelfformulationsincludeallaminoaciddeficientandmethioninedeficient.Selectaminoaciddeficienciesmaybecustomordered.
Description:
Selenomethionineincorporationisastandardmethodfordeterminingthephasesinproteincrystallographybysingle-ormultiwavelengthanomalousdispersion.Tosupporttheeffortsofscientistsusingthismethodology,ExpressionSystemssuppliesaMethionineDeficientmediumformulation.Se-Metincorporationlevelsarereportedtobetypically75%(ProteinScience16(9):2023-9 · October2007)butcanrangefrom70-100%usingthismedium.
StructuralBIOLOGistsareincreasinglyutilizingNMRspectroscopytoobtaininformationaboutthestructureanddynamicsofproteinsandtheircomplexes.Thesimplestandmostcost-effectivebiosyntheticlabelingmethodforproteinsolid-stateNMRistouniformlylabelallcarbonandnitrogenatomswith13Cand15N.ExpressionSystemsstocksanallaminoaciddeficientvariantwithintheDeltaseriestoenablescientistsinterestedinaddingasourceofisotopically-labeledaminoacids.Customformulationsmayalsoberequestedspecifyingaformulationdeficientincarbonsourcesorspecificaminoacids.Customformulationsrequirea20Lminimumorderandaredeliveredinapproximately6weeks.
Specification:
Mediatype | Serum-freeliquid,1X |
Platformcompatibility | FunctionswithBestBac™,Bacmid,Bac-to-Bac®,BacMam™,MultiBac™andotherBEVSplatforms |
Celllines | Spodopterafrugiperda(Sf9,Sf21),Trichoplusiani(TniandHighFive™),andDrosophiliamelanogaster(S2) |
Shippingcondition | Ambient |
Storagecondition | 2-8⁰C,protectfromlight |
Use-bydate | Oneyearfromdateofmanufacture |