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ESF921™InsectCellCultureMediumisacompleteserum-free,protein-freemediumdevelopedforrobustcellgrowth,proteinproductionandbaculovirusvectorproductionforawiderangeofinsectcellsincludingSf9,Sf21,Tni(HighFive™),andDrosophilaS2.ESF921containsL-GlutamineandKolliphor®P188(PluronicF68).
Description:
WidelyrecognizedastheindustrystandardforBEVSapplications,ExpressionSystemsinsectcellculturemediaaredesignedformaximumvirusandproteinexpression.Thisserum-free,protein-freeinsectcellculturemediumprovidesunmatchedcellgrowthandexpressionforawiderangeofinsectcellsincludingSf9,Sf21,Tni(HighFiveTM)andDrosophilaS2.
- Sf9celldensitiesof15x106cells/mlorgreater
- Produceshightiterbaculovirusvectoryieldsof5E8to3E9infectiousunits/ml
- Availablein1Lbottles,bulkpackagingandsingle-usebagsthateliminatetheneedtotransfermedia
- Complete,ready-to-use,containsL-glutamine
- Idealforscale-upmanufacturing
- Workswithawidevarietyofbaculovirusandhostcellplatforms
- Instockforimmediateshipment
- Adaptedcellsavailable
SuperiorGrowthandReproducibility
ExpressionSystems’ESF921TMInsectCellCultureMediumisdesignedformaximumexpressionforapplicationssuchas;genetherapydrugsubstance,virus-likeparticles,virusvectorsandproteinsofinterest.ESF921TMiswidelyrecognizedastheindustrystandardforthebaculovirusandinsecthostcellsystem.ThelinegraphpresentsreproducIBLegrowthcurvesforSf9cellculturesinninedifferentlotsofESF921TMmediummanufacturedoveraperiodof12months.
Sf9cellsfromamid-logphaseculturewereseededinto*9differentlotsofESF921TMatadensityof0.75×10,6/ml.Culturesweresampleddailytodetermineviablecellcount.
Specification:
Mediatype | Ready-to-useserum-freeliquid,1X |
Platformcompatibility | FunctionswithBestBac™,Bacmid,Bac-to-Bac®,BacMam™,MultiBac™andotherBEVSplatforms |
Celllines | Spodopterafrugiperda(Sf9,Sf21),Trichoplusiani(TniandHighFive™)andDrosophilamelanogaster(S2) |
Use-bydate | Oneyearfromdateofmanufacture |